A novel secreted protein, NISP1, is phosphorylated by soybean Nodulation Receptor Kinase to promote nodule symbiosis  

作　　者：Baolan Fu Zhipeng Xu Yutao Lei Ru Dong Yanan Wang Xiaoli Guo Hui Zhu Yangrong Cao Zhe Yan 

机构地区：[1]State Key Lab of Agricultural Microbiology,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China [2]State Key Lab of Agricultural Microbiology,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [3]National Key Facility for Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing100081,China

出　　处：《Journal of Integrative Plant Biology》2023年第5期1297-1311,共15页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (32090063 to Y.C.);the National Key R&D Program of China (2016YFD0100702 to Y.C.);the Natural Science Foundation of Hubei Province (2020CFA008 to Y.C.);a Fundamental Research Fund for the Central Universities (2662022SKYJ002 to Y.C.)。

摘　　要：Nodulation Receptor Kinase(NORK) functions as a co-receptor of Nod factor receptors to mediate rhizobial symbiosis in legumes, but its direct phosphorylation substrates that positively mediate root nodulation remain to be fully identified.Here, we identified a GmNORK-Interacting Small Protein(GmNISP1) that functions as a phosphorylation target of GmNORK to promote soybean nodulation. GmNORKα directly interacted with and phosphorylated GmNISP1. Transcription of GmNISP1 was strongly induced after rhizobial infection in soybean roots and nodules. GmNISP1 encodes a peptide containing 90 amino acids with a “DY” consensus motif at its N-terminus.GmNISP1 protein was detected to be present in the apoplastic space. Phosphorylation of GmNISP1 by GmNORKα could enhance its secretion into the apoplast. Pretreatment with either purified GmNISP1 or phosphorylation-mimic GmNISP1~(12D) on the roots could significantly increase nodule numbers compared with the treatment with phosphorylation-inactive GmNISP1~(12A).The data suggested a model that soybean GmNORK phosphorylates GmNISP1 to promote its secretion into the apoplast, which might function as a potential peptide hormone to promote root nodulation.

关 键 词：legume-rhizobia symbiosis Nodulation Receptor Kinase protein phosphorylation root nodule symbiosis SOYBEAN 

分 类 号：S565.1[农业科学—作物学]