烟草NtHDZIV8基因在腺毛发育中的功能验证  

Functional Verification of NtHDZIV8 Gene in the Development of Glandular Trichomes in Tobacco

作　　者：崔露莹 陈明丽[1] 余文 李志远 巫升鑫 李东徽 盖梦琦 解敏敏[1] 龚达平[1] 程崖芝 CUI Luying;CHEN Mingli;YU Wen;LI Zhiyuan;WU Shengxin;LI Donghui;GAI Mengqi;XIE Minmin;GONG Daping;CHENG Yazhi(Institute of Tobacco Research of CAAS,Qingdao 266101,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China;Institute of Tobacco Science Research,Fujian Tobacco Monopoly Administration,Fuzhou 350003,China;Shiyan Tobacco Company of Hubei Province,Shiyan,Hubei 442000,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]中国农业科学院研究生院,北京100081 [3]福建省烟草专卖局烟草科学研究所,福州350003 [4]湖北省烟草公司十堰市公司,湖北十堰442000

出　　处：《中国烟草科学》2023年第2期7-14,共8页Chinese Tobacco Science

基　　金：中央级公益性科研院所基本科研业务费专项(1610232022009);中国烟草总公司福建省公司科技计划项目(2022350000240090);中国烟草总公司科技重大专项(110202001023 JY-06)。

摘　　要：为验证NtHDZIV8在腺毛发育过程中的功能,在红花大金元中克隆了NtHDZIV8基因并进行生物信息学分析,通过荧光定量PCR(qRT-PCR)探究了NtHDZIV8的表达模式,进一步利用亚细胞定位与病毒诱导的基因沉默(VIGS)技术初步验证了NtHDZIV8基因对烟草腺毛发育的调控作用。结果表明,NtHDZIV8进化高度保守,具有典型的HD-LZ、START和SAD结构域,属于HDZIP IV家族。表达模式分析表明,NtHDZIV8在腺毛中高表达,并受植物激素NAA、GA3和MeJA的诱导。亚细胞定位结果显示NtHDZIV8定位于细胞核。病毒诱导本氏烟中NbHDZIV8基因沉默导致长柄腺毛的柄细胞基部膨大,而不影响短柄腺毛形态和腺毛密度。该研究表明NbHDZIV8对烟草腺毛形态具有调控作用,为解析烟草腺毛发生发育调控机制奠定了理论基础。To verify the function of NtHDZIV8 in the development of glandular trichomes,the coding sequence(CDS)of NtHDZIV8 was cloned from Honghuadajinyuan and further used in bioinformatics analysis.The expression pattern of NtHDZIV8 was analyzed by qRT-PCR,and the role of NtHDZIV8 in development of glandular trichomes was verified using subcellular localization and virus-induced gene silencing(VIGS).The results showed that NtHDZIV8 was highly conserved and contained typical HD-LZ,START and SAD domains,belonging to the HD-ZIP IV family.The expression pattern analysis showed that NtHDZIV8 was highly expressed in glandular trichomes,and was induced by plant hormones GA3,NAA and MeJA.Subcellular localization results showed that NtHDZIV8 was located in the nucleus.The results of VIGS analysis showed that NbHDZIV8 gene regulated basal expansion of stalk cells in long glandular trichomes,but did not affect the morphology and density of short glandular trichomes in N.benthamiana.The results in this study demonstrate that NtHDZIV8 regulates the development of long glandular trichomes in tobacco,which provides a theoretical basis for the analysis of the regulatory mechanism of tobacco trichome development.

关 键 词：烟草 HDZIV8 腺毛 基因沉默 

分 类 号：S572.03[农业科学—烟草工业]