烟草镰刀菌根腐病抗性鉴定方法研究  

Study on Identification of Tobacco Variety Resistance to Fusarium oxysporum

作　　者：李雪君[1] 郭敬 孙计平[1] 孙焕[1] 李芳芳[2] 平文丽[1] 李建华[3] 俎焕新[1] 李旭辉[1] 侯咏[1] 耿胜娜 LI Xuejun;GUO Jing;SUN Jiping;SUN Huan;LI Fangfang;PING Wenli;LI Jianhua;ZU Huanxin;LI Xuhui;HOU Yong;GENG Shengna(Tobacco Research Institute of Henan Academy of Agricultural Sciences,Key Laboratory of Green Prevention and Control of Tobacco Diseases and Pests in Huanghuai Tobacco Area of Tobacco Industry,Xuchang,Henan 461000,China;Henan Province Tobacco Company,Zhengzhou 450018,China;Henan Xuchang Tobacco Company,Xuchang,Henan 461000,China)

机构地区：[1]河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南许昌461000 [2]河南省烟草公司,郑州450018 [3]河南省烟草公司许昌市公司,河南许昌461000

出　　处：《中国烟草科学》2023年第2期43-51,共9页Chinese Tobacco Science

基　　金：中国烟草总公司河南省公司科技项目(2018410000270099);河南省烟草公司许昌市公司科技项目(2018411301240126)。

摘　　要：为获得不同烟草品种镰刀菌根腐病抗性的鉴定方法,采用镰刀菌粗毒素幼芽接种、孢子悬浮液培养、带菌菌谷接种、离体叶片菌饼接种、草酸局部注射等5种方法对4个品种(小黄金1025、中烟100、长脖黄、664-01)的镰刀菌抗病性进行了研究。结果表明,镰刀菌毒素液胁迫法可以鉴定品种的抗感程度,适宜体系毒素浓度为8%,处理时间6 d;镰刀菌孢子悬浮液接种法可以鉴定品种的抗感程度,适宜体系孢子液浓度为107个/mL,处理时间4 d;带菌菌谷接种也可以鉴定品种的抗感程度,适宜体系菌谷比例为20%,处理时间9 d;离体叶片用菌饼和草酸液处理,能区分品种的抗感性,但不能区分品种的抗感程度。研究结果认为孢子悬浮液接种法和带菌菌谷接种与其他3种方法相比可以准确评价品种对镰刀菌根腐病的抗感性。To obtain suitable identifying methods of tobacco root rot resistance in different tobacco varieties(Xiaohuangjin1025,Zhongyan100,Changbohuang,664-01),the inoculation crude toxin of tobacco seedlings,spore suspension culture,inoculation of grain culture with fungi,inoculation mycelial cake of detached leaf and local injection of oxalic acid were investigated.The results showed that crude toxin stress could identify the resistance degree of tobacco varieties,and the suitable system was treated within 6 d at 8%toxin concentration.The inoculation spore suspension could identify the degree of resistance of different varieties and the suitable system was treated within 4 d and with 107 spores per mL.The inoculation of grain culture with fungi could also identify the degree of resistance of tobacco varieties,and the suitable system was treated within 9 d at the toxin concentration of 20%pathogenic fungus grain culture.The treatment of detached leaf with mycelial cake oxalic acid could distinguish the resistance of varieties,but could not differentiate the degree of resistance.The results showed that the spore suspension inoculation method and the fungal wheat culture inoculation method could accurately evaluate the resistance of varieties to Fusarium root rot compared with the other three methods.

关 键 词：烟草 镰刀菌根腐病 毒素 孢子液 菌饼 抗病性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]