云南耿马甘蔗宿根矮化病的发生分布和病原分子检测  

Occurrence and Distribution of Sugarcane Ratoon Stunting Disease and Molecular Detection of its Pathogen in Gengma,Yunnan

作　　者：马永德 王晓燕[2] 李银煳 李婕 张荣跃[2] 单红丽[2] 黄应昆 MA Yongde;WANG Xiaoyan;LI Yinhu;LI Jie;ZHANG Rongyue;SHAN Hongli;HUANG Yingkun(Agricultural and Rural Affairs Bureau of Gengma Country,Gengma,Yunnan 677500;Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Kaiyuan,Yunnan 661699)

机构地区：[1]耿马县农业农村局,云南耿马677500 [2]云南省农业科学院甘蔗研究所,云南开远661699

出　　处：《甘蔗糖业》2023年第2期10-15,共6页Sugarcane and Canesugar

基　　金：财政部和农业农村部国家现代农业产业技术体系专项资金(CARS-170303);云岭产业技术领军人才培养项目(2018LJRC56);云南省现代农业产业技术体系建设专项资金资助(YNGZTX-4-92)。

摘　　要：为摸清云南耿马甘蔗宿根矮化病的发生分布,为甘蔗温水脱毒健康种苗生产和推广应用提供科学依据,本研究对云南耿马不同甘蔗品种植期的80个样本进行宿根矮化病菌分子检测。结果表明:61个样本为阳性,阳性检出率76.25%,其中强阳性样本34个,占42.50%;10个主栽或主推品种均发病,发病率在37.50%~100%之间,以粤糖93-159、粤糖83-88、云瑞07-1433、盈育91-59、柳城05-136发病最重,发病率为77.27%~100%,强阳性率为44.44%~66.67%,其次是新台糖22号、云瑞06-189、云瑞10-328、云蔗05-51和云蔗08-1609,发病率为37.50%~100%,强阳性率为0%~14.29%;植期上1~5年均不同程度发病,但无明显的规律性差异。本研究检测表明甘蔗宿根矮化病已在云南耿马蔗区普遍发生且危害严重,急需生产和推广种植甘蔗温水脱毒健康种苗,以促进蔗糖产业的可持续高质量发展。In order to ascertain the occurrence and distribution of sugarcane ratoon stunting disease(RSD)in Gengma,Yunnan and provide scientific basis for the production and application of virus-free healthy seedlings treated with hot-water,80 samples collected from different planting periods and varieties in Gengma,Yunnan were detected by molecular detection method.The results showed that 61 samples(76.25%out of 80)were RSD positive,of which 34 samples(42.50%out of 80)were strongly RSD positive.Ten main cultivars and popularized varieties were all infected,with the incidence rate of RSD was between 37.50%~100%.Yuetang93-159,Yuetang83-88,Yunrui07-1433,Yingyu91-59,Liucheng05-136 were the most infected seriously,with the incidence rate was between 77.27%~100%and strongly positive rate was between 44.44%~66.67%.Followed by ROC22,Yunrui06-189,Yunrui10-328,Yunzhe05-51 and Yunzhe08-1609,with the incidence rate was between 37.50%~100%and strongly positive rate was between 0%~14.29%.RSD occurred in different degrees from 1 to 5 years during the planting period,but there was no significant regular difference.The detection of this study indicated that RSD has occurred widely and seriously in Gengma planting areas of Yunnan. It is urgent to produce and apply the sugarcane virus-free healthy seedlings treated with hot-water toensure the sustainable and high-quality development of sugar industry.

关 键 词：云南耿马 甘蔗宿根矮化病 发生分布 分子检测 

分 类 号：S566.1[农业科学—作物学]