吡咯腙对溶酶体中Hg^(2+)的荧光成像  

Pyrrole-based hydrazone for fluorescent imaging of Hg^(2+)in lysosomes

作　　者：王震 李思媛 王元 吴伟娜 张磊 陈忠 闫玲玲 WANG Zhen;LI Si‑Yuan;WANG Yuan;WU Wei‑Na;ZHANG Lei;CHEN Zhong;YAN Ling‑Ling(College of Chemistry and Chemical Engineering,Henan Polytechnic University,Jiaozuo,Henan 454000 China;School of Materials and Mechanical and Electrical Engineering,Jiangxi Science and Technology Normal University,Nanchang 330013 China;School of Physics and Electronic Information Engineering,Henan Polytechnic University,Jiaozuo,Henan 454000 China)

机构地区：[1]河南理工大学化学化工学院,焦作454000 [2]江西科技师范大学材料与机电学院,南昌330013 [3]河南理工大学物理与电子信息学院,焦作454000

出　　处：《无机化学学报》2023年第6期1122-1130,共9页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21907023,21001040,61964008);河南省重点研发与推广专项科技攻关项目(No.232102320092);江西省自然科学基金(No.20212BAB204017);河南省高校基本科研业务费项目(No.NSFRF230402);河南理工大学“双一流”创建工程项目(No.AQ20230745,AQ20230754);河南理工大学研究生教改项目(No.2021YJ16)资助。

摘　　要：合成了一种新的吡咯腙探针1,用于Hg^(2+)的比色和荧光开启检测。探针1对Hg^(2+)的检测限为45 nmol·L^(-1),缔合常数为5.78×10^(8)L·mol^(-1)。值得注意的是,工作p H范围为4.0~10.0。Job曲线和MS数据证实探针与Hg^(2+)形成1:1的配合物。通过1H NMR、时间分辨荧光光谱和密度泛函理论(DFT)计算系统研究了探针与Hg^(2+)的配位模式。此外,由于吗啉基团的存在,探针可以检测He La细胞溶酶体中的Hg^(2+)。A novel pyrrole-based hydrazone has been synthesized for colorimetric and fluorescent turn-on detection of Hg^(2+)ions.A 1:1 binding ratio of the Hg^(2+)complex has been obtained from Job's plots and MS data.The coordination mode was systematically investigated by 1H NMR,time-resolved fluorescence spectroscopy,and density functional theory(DFT)calculations.The limit of detection of 1 for Hg^(2+)was as low as 45 nmol·L^(-1)with an association constant of 5.78×10^(8)L·mol^(-1).It is worth noting that the response of 1 to Hg^(2+)was good in a pH range of 4.0 to 10.0.In addition,owing to the existence of the morpholine group,the probe can detect lysosomal Hg^(2+)in HeLa cells.

关 键 词：细胞成像 荧光探针 Hg^(2+) 腙 溶酶体 吡咯 

分 类 号：O614.243[理学—无机化学]