Comparative analysis of the gut microbiota of wild adult rats from nine district areas in Hainan,China  

作　　者：Li-Na Niu Guan-Nan Zhang Duan-Duan Xuan Chong Lin Zi Lu Pei-Pei Cao Shao-Wen Chen Yong Zhang Xiu-Ji Cui Shou-Kui Hu 

机构地区：[1]Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Medical University,Haikou 571199,Hainan Province,China [2]The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases,Hainan Medical University,Haikou 571199,Hainan Province,China [3]Department of Pathogen Biology,Hainan Medical University,Haikou 571199,Hainan Province,China [4]Department of Laboratory,Xinxiang First People's Hospital,Xinxiang 453000,Henan Province,China [5]Department of Laboratory,The Second Affiliated Hospital of Hainan Medical University,Haikou 570311,Hainan Province,China [6]Department of Clinical Laboratory,Peking University Shougang Hospital,Beijing 100144,China

出　　处：《World Journal of Gastroenterology》2023年第22期3469-3481,共13页世界胃肠病学杂志（英文版）

基　　金：Supported by Hainan Province Science and Technology Special Fund,No.ZDYF2022SHFZ114;Hainan Provincial Natural Science Foundation of China,No.820RC650;National Natural Science Foundation of China,No.82060377;Innovative Research Project for Graduate Students of Hainan Medical University,No.HYYS2020-18,No.HYYS2021A09,and No.HYYS2021A22.

摘　　要：BACKGROUND Wild rats have the potential to hold zoonotic infectious agents that can spread to humans and cause disease.AIM To better understand the composition of gut bacterial communities in rats is essential for preventing and treating such diseases.As a tropical island located in the south of China,Hainan province has abundant rat species.Here,we examined the gut bacterial composition in wild adult rats from Hainan province.METHODS Fresh fecal samples were collected from 162 wild adult rats,including three species(Rattus norvegicus,Leopoldamys edwardsi,and Rattus losea),from nine regions of Hainan province between 2017-2018.RESULTS We analyzed the composition of gut microbiota using the 16S rRNA gene amplicon sequencing.We identified 4903 bacterial operational taxonomic units(30 phyla,175 families,and 498 genera),which vary between samples of different rat species in various habitats at various times of the year.In general,Firmicutes were the most abundant phyla,followed by Bacteroidetes(15.55%),Proteobacteria(6.13%),and Actinobacteria(4.02%).The genus Lactobacillus(20.08%),unidentified_Clostridiales(5.16%),Romboutsia(4.33%),unidentified_Ruminococcaceae(3.83%),Bacteroides(3.66%),Helicobacter(2.40%)and Streptococcus(2.37%)were dominant.CONCLUSION The composition and abundance of the gut microbial communities varied between rat species and locations.This work provides fundamental information to identify microbial communities useful for disease control in Hainan province.

关 键 词：HAINAN Rat Fecal microbiome Microbial community 16S rRNA 

分 类 号：R446.5[医药卫生—诊断学]