超高效液相色谱串联三重四极杆质谱法测定药用铝箔中34种芳香伯胺  

Determination of 34 primary aromatic amines in medicinal aluminum foil by ultra performance liquid chromatography tandem triple quadrupole mass spectrometry

作　　者：邹佳佳 杨乾展 代磊 李舒雅 何丹[1] ZOU Jiajia;YANG Qianzhan;DAI Lei;LI Shuya;HE Dan(College of Pharmacy,Chongqing Medical University,Chongqing,400016,China;Shimadzu Co.,LTD,Analytical&Measuring Instruments Division,Chongqing,400010,China)

机构地区：[1]重庆医科大学药学院,重庆400016 [2]岛津企业管理有限公司分析计测事业部,重庆400010

出　　处：《环境化学》2023年第5期1763-1766,共4页Environmental Chemistry

基　　金：重庆医科大学药学院支部书记“双带头人”项目(YXY2021SDTR01)资助.

摘　　要：本文建立了超高效液相色谱串联三重四极杆质谱法(UPLC-MS/MS)检测药用铝箔类中34种芳香伯胺的含量.采用Waters ACQUITY UPLC-HSS T3(100 mm×2.1 mm I.D.,1.8μm)色谱柱,以0.1%甲酸水溶液-乙腈为流动相,梯度洗脱,流速为0.4 mL·min^(−1),柱温为40℃.采用电喷雾离子源(ESI+),工作模式为多反应监测模式(MRM).34种芳香伯胺在1—100 ng·mL^(−1)浓度范围内均具有较好的线性关系(r>0.999),各化合物保留时间和峰面积的相对标准偏差均小于0.21%和5.00%,加标回收率在73.5%-114.8%之间.该方法具有灵敏度高、重复性好、准确度高的特点,可用于药用铝箔中34种芳香伯胺的测定.An ultra performance liquid chromatography tandem triple quadrupole mass spectrometry(UPLC-MS/MS)method was developed for the determination of 34 primary aromatic amines in medicinal aluminum foils.Waters ACQUITY UPLC-HSS T3(100 mm×2.1 mm I.D.,1.8μm)chromatographic column was used,with 0.1%formic acid aqueous solution-acetonitrile as mobile phase and gradient elution.The flow rate was 0.4 mL·min^(−1),column temperature was 40℃.Electrospray ion source(ESI+)was used,and the working mode was multi-reaction monitoring mode(MRM).The 34 primary aromatic amines had satisfactory linear relationships in the concentration range of 1—100 ng·mL^(−1)(r>0.999).The relative standard deviations of retention time and peak area of each compound were less than 1.21%and 5.00%,respectively.The recoveries were between 73.5%and 114.8%.The established method has the characteristics of high sensitivity,good repeatability and high accuracy,and is feasible for the determination of 34 primary aromatic amines in medicinal aluminum foil.

关 键 词：芳香伯胺 药用铝箔 UPLC-MS/MS 

分 类 号：O657.63[理学—分析化学] TQ460.7[理学—化学]