Thymoquinone enhances the antioxidant and anticancer activity of Lebanese propolis  

作　　者：Sima AlDreini Zaynab Fatfat Najwa Abou Ibrahim Maamoun Fatfat Hala Gali-Muhtasib Hala Khalife 

机构地区：[1]Rammal Rammal Laboratory(ATAC Group),Faculty of Sciences I,Hadath 1003,Lebanon [2]Department of Biology,American University of Beirut,Beirut 1107-2020,Lebanon [3]Applied Biochemistry Laboratory,School of Pharmacy,Camerino University,Camerino 62032,Italy

出　　处：《World Journal of Clinical Oncology》2023年第5期203-214,共12页世界临床肿瘤学杂志（英文版）

基　　金：Supported by the Lebanese University and the Undergraduate Research Experience funds at the American University of Beirut.

摘　　要：BACKGROUND Reactive oxygen species(ROS)are produced by multiple cellular processes and are maintained at optimal levels in normal cells by endogenous antioxidants.In recent years,the search for potential exogenous antioxidants from dietary sources has gained considerable attention to eliminate excess ROS that is associated with oxidative stress related diseases including cancer.Propolis,a resinous honeybee product,has been shown to have protective effects against oxidative stress and anticancer effects against several types of neoplasms.AIM To investigate the antioxidant and anticancer potential of Lebanese propolis when applied alone or in combination with the promising anticancer compound Thymoquinone(TQ)the main constituent of Nigella sativa essential oil.METHODS Crude extracts of Lebanese propolis collected from two locations,Rashaya and Akkar-Danniyeh,were prepared in methanol and the total phenolic content was determined by Folin–Ciocalteu method.The antioxidant activity was assessed by the ability to scavenge 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical and to inhibit H2O2-induced oxidative hemolysis of human erythrocytes.The anticancer activity was evaluated by[3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide]MTT assay against HCT-116 human colorectal cancer cells and MDAMB-231 human breast cancer cells.RESULTS The total phenolic content of propolis extract from Rashaya and Akkar-Danniyeh were 56.81μg and 83.503μg of gallic acid equivalent/mg of propolis,respectively.Both natural agents exhibited strong antioxidant activities as evidenced by their ability to scavenge DPPH free radical and to protect erythrocytes against H2O2-induced hemolysis.They also dose-dependently decreased the viability of both cancer cell lines.The IC50 value of each of propolis extract from Rashaya and Akkar-Danniyeh or TQ was 22.3,61.7,40.44μg/mL for breast cancer cells at 72 h and 33.3,50.9,33.5μg/mL for colorectal cancer cells at the same time point,respectively.Importantly,the inhibitory effects of propol

关 键 词：Lebanese propolis THYMOQUINONE COMBINATION Antioxidant activity Anticancer activity Phenolic compounds 

分 类 号：R285[医药卫生—中药学]