鲑鱼精DNA-阳离子表面活性剂体系的共振光散射光谱分析及应用  

Resonance Light Scattering Spectroscopy of Salmon Sperm DNA-Cationic Surfactant System and Its Application

作　　者：邹明静[1] 董懿萱 穆昀 伏梦瑶 楚琳[1] 郭雨晴 崔一帆 泮雨硕 ZOU Ming-jing;DONG Yi-xuan;MU Yun;FU Meng-yao;CHU Lin;GUO Yu-qing;CUI Yi-fan;PAN Yu-shuo(Central Laboratory of Heze Medical College,Heze Shandong 274030,China)

机构地区：[1]菏泽医学专科学校中心实验室,山东菏泽274030

出　　处：《当代化工》2023年第4期771-774,共4页Contemporary Chemical Industry

基　　金：山东省医药卫生科技发展计划项目(项目编号:202011001344);菏泽医学专科学校科研项目(项目编号:H20Z01)。

摘　　要：鲑鱼精DNA与阳离子表面活性剂十四烷基二甲基苄基氯化铵(TDMBA)通过静电作用可结合形成缔合物微粒,通过Zeta电位分析、动态光散射、紫外光谱和共振光散射光谱对形成的缔合物微粒进行了表征,共振光散射光谱显示其在620 nm处出现较强的共振光散射峰,且其散射光强度随鲑鱼精DNA质量浓度的增加线性增强,可用于检测0.3~667 ng·mL^(-1)的鲑鱼精DNA,检出限为0.2 ng·mL^(-1),此法可用于DNA的定量分析,简单快速、灵敏且选择性好,测定结果较好。Salmon sperm DNA and cationic surfactant tetradecyl dimethyl benzyl ammonium chloride(TDMBA)can combine to form association particles through electrostatic interaction.Zeta potential analysis,dynamic light scattering,UV spectrum and resonance light scattering spectrum were used to characterize the association particles.Resonance scattering spectrum showed a strong resonance light scattering peak at 620 nm,and its scattering light intensity increased linearly with the increasing of mass concentration of salmon sperm DNA.It can be used to detect salmon sperm DNA of 0.3~667 ng·mL^(-1)with a detection limit of 0.2 ng·mL^(-1).Owing to its simple,rapid,sensitive and selective,this method can be used for quantitative analysis of DNA with satisfactory results.

关 键 词：鲑鱼精DNA 十四烷基二甲基苄基氯化铵 缔合物微粒 共振光散射 

分 类 号：O657.3[理学—分析化学]