HDAC2基因多态性与噪声性听力损失易感性的关联  

Association between polymorphism of HDAC2 gene and susceptibility to noise-induced hearing loss(NIHL)in Hanpopulation

作　　者：徐娅冬 李雅丽 黄魏宁[2] XU Yadong;LI Yali;HUANG Weining(Department of Otolaryngology,the First People's Hospital of Dongcheng District,Beijing 100075,China;Department of Otolaryngology,Beijing Hospital of Health,Beijing 100005,China)

机构地区：[1]北京市东城区第一人民医院耳鼻喉科,北京100075 [2]卫生部北京医院耳鼻喉科,北京100005

出　　处：《新疆医科大学学报》2023年第5期668-673,678,共7页Journal of Xinjiang Medical University

基　　金：北京市卫健委科技计划项目(202007)。

摘　　要：目的 研究组蛋白去乙酰化酶2(HDAC2)基因多态性与噪声性听力损失(Noise-induced hearing loss, NIHL)易感性的关联。方法 以噪声作业工人为研究对象,选取2019年6月-2022年6月北京市东城区第一人民医院耳鼻喉科确诊的246例NIHL患者为观察组,选择同期246例听力未损伤者为对照组,均接受纯音听力检测,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测外周血HDAC2基因多态性(SNP)位点,采用Logistic回归分析法明确HDAC2基因SNP位点与NIHL易感性的关系。结果 与对照组比较,观察组高频听阈升高,差异有统计学意义(P<0.05)。观察组与对照组HDAC2基因rs10499080和rs6568819位点基因型分布比较,差异有统计学意义(P<0.05)。与对照组比较,观察组rs10499080和rs6568819位点T等位基因频率升高,差异有统计学意义(P<0.05)。Logistic分析表明rs12208304、rs1320445和rs3757016位点各基因型患者NIHL的发生风险无明显增加(P>0.05);rs10499080位点TT基因型[OR(95%CI)=1.659(1.120~2.694)]及隐性模型CT+TT基因型[OR(95%CI)=1.944(1.120~3.217)]NIHL风险增加(P<0.05),显性模型CC+CT基因型[OR(95%CI)=0.731(0.535~0.917)]NIHL风险降低(P<0.05);rs6568819位点患者TT基因型[OR(95%CI)=3.108(1.531~8.731)]及隐性模型CT+TT基因型[OR(95%CI)=1.510(1.031~2.536)]NIHL风险增加(P<0.05),显性模型CC+CT基因型[OR(95%CI)=0.892(0.420~0.954)]NIHL风险降低(P<0.05)。结论 HDAC2基因rs10499080和rs6568819位点碱基C突变为T的噪声作业工人发生NIHL的风险较高。Objective To explore the association between polymorphisms ofhistone deacetylase 2(HDAC2)gene and susceptibility to noise-induced hearing loss(NIHL)in Han population.Methods The Han population noise operation workers were used as the study subjects,246 patients with NIHL diagnosed in the First People's Hospital of Dongcheng District from June 2019 to June 2022 were selected as observation group,and 246 hearing unimpaired patients during the same period were selected as control group.All of the patients were received pure tone hearing test,and the peripheral blood HDAC2 gene polymorphism(SNP)loci were detected by polymerase chain reaction-restriction fragment length polymorphism(PCRRFLP).The association between HDAC2 gene SNP loci and NIHL susceptibility was clarified by logistic regression analysis.Results Compared with control group,the high-frequency hearing threshold in observation group was increased,the difference was statistically significant(P<0.05).The genotype distribution of rs10499080 and rs6568819 loci of HDAC2 gene in observation group and control group was statistically different(P<0.05);and the frequency of T allele at rs10499080 and rs6568819 sites in observation group was significantly higher than that in control group(P<0.05).Logistic analysis revealed that the patients with each genotype at rs12208304,rs1320445 and rs3757016 loci had no significantly increased risk of NIHL(P>0.05),while the risk of NIHL was increased for TT genotype[OR(95%CI)=1.659(1.120 to 2.694)]and recessive model CT+TT genotype[OR(95%CI)=1.944(1.120 to 3.217)]at rs10499080 locus(P<0.05);and dominant model CC+CT genotype[OR(95%CI)=0.731(0.535 to 0.917)]NIHL risk was reduced(P<0.05).Increased risk of NIHL in patients with TT genotype[OR(95%CI)=3.108(1.531 to 8.731)]and recessive model CT+TT genotype[OR(95%CI)=1.510(1.031 to 2.536)]at rs6568819 locus(P<0.05),and the dominant model CC+CT genotype[OR(95%CI)=0.892(0.420~0.954)]had a decreased risk of NIHL(P<0.05).Conclusion Noise-working workers in Han population with base C muta

关 键 词：噪声性听力损失 组蛋白去乙酰化酶2 基因多态性 易感性 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]