果王素下调肿瘤坏死因子α和血小板源性生长因子B减轻大鼠放射性肺损伤  

Kiwi fruit essence reduces radiation-induced lung injury by down-regulating TNF-αand PDGF-B in rats

作　　者：刘理静 钱红 贺立洋 魏文婕 周美玲 贺兼斌[2] LIU Lijing;QIAN Hong;HE Liyang;Wei Wenjie;ZHOU Meiling;HE Jianbin(Department of Clinical Medicine,School of Medieine,Changsha Social Work College,Changsha 410004;Department of Respiratory and Critical Care Medicine,The First People's Hospital of Huaihua,Huaihua 418000,China)

机构地区：[1]长沙民政职业技术学院医学院临床医学系,湖南长沙410004 [2]怀化市第一人民医院呼吸与危重症医学科,湖南怀化418000

出　　处：《细胞与分子免疫学杂志》2023年第4期332-338,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：怀化市科技计划(2017G3301);湖南省自然科学基金(2018JJ6045);湖南省卫生与健康委员会资助科研项目(B20180064)

摘　　要：目的观察肿瘤坏死因子α(TNF-α)和血小板源性生长因子B(PDGF-B)在果王素减轻大鼠放射性肺损伤(RILI)中的作用.方法96只雄性健康SD大鼠,随机分为空白对照组、模型组、(60、240 mg/kg)果王素处理组,各组24只.后3组大鼠利用6 MV的X射线18 Gy分次照射全肺,构建RILI动物模型,照射后次日,后2组大鼠分别给予60 mg/kg、240 mg/kg果王素灌胃,而以9 g/L氯化钠溶液灌胃前2组大鼠,每天均1次.后3组分别于第14、28、56天随机处死8只大鼠,空白对照组在第56天全部处死作为总体对照.收集血液并分离,ELISA检测血清中TNF-α和PDGF-B含量.留取肺组织,HE和Masson染色评价肺泡炎及肺纤维化(PF)情况,测定羟脯氨酸(HYP)含量,实时定量PCR和免疫组织化学染色法分别检测肺组织中TNF-α、PDGF-B mRNA及蛋白表达.结果与同期模型组相比,(60、240)mg/kg果王素处理组14 d、28 d肺泡炎和28 d、56 d PF病变显著减轻.与空白对照组比较,模型组28 d、56 d肺组织HYP含量增加,而各时间点血清及肺组织TNF-α和PDGF-B表达升高.与模型组同期相比,(60、240)mg/kg果王素处理组14 d、28 d血清和肺组织TNF-α表达下调,28 d、56 d肺组织HYP含量以及血清和肺组织PDGF-B表达降低.并且,240 mg/kg果王素处理组同期上述指标较60 mg/kg果王素处理组下降.结论果王素能减轻大鼠RILI,与其早期下调TNF-α表达和中晚期降低PDGF-B水平有关.Objective To observe the role of tumor necrosis factor-α(TNF-α)and platelet-derived growth factor-B(PDGF-B)in kiwi fruit essence-mediated protection of radiation-induced lung injury(RILI)in rats.Methods 96 male healthy Sprague-Dawley rats were divided into normal control group,model group,and kiwi fruit essence treatment group(60 and 240 mg/kg)by the random number table method,with 24 animals in each group.The whole lungs underwent 6 MV X-ray iradiation(18 Gy)to induce RILI animal models in rats of the latter three groups.On the next day after irradiation,rats in the latter two groups were intragastrically administrated with 60 or 240 mg/kg kiwi fruit essence,once a day.The rats in the normal control and model groups were treated with 9 g/L sodium chloride solution.Eight rats in the latter three groups were randomly sacrificed on days 14,28,and 56,while normal control rats were sacrificed on day 56 as the overall control.Blood samples were collected and separated.Serum concentrations of TNF-αand PDGF-B were detected using ELISA.The lung tissues were isolated for HE and Masson staining to evaluate alveolitis and pulmonary fibrosis(PF).The hydroxyproline(HYP)content in lung tissues was detected.The mRNA and protein expression of pulmonary TNF-αand PDGF-B were determined by quantitative real-time PCR and immunohistochemistry.Results Compared with the model group,treatment with 60 and 240 mg/kg kiwi fruit essence group significantly reduced alvelitis on days 14 and 28 as well as PF lesions on days 28 and 56.Compared with the normal control group,HYP content in the lung tissue of the model group increased on day 28 and day 56,while TNF-αand PDGF-B levels in the serum and lung tissues increased at each time point.Compared with the model group during the same period,60 and 240 mg/kg kiwi fruit essence element treatment group reported the diminished levels of serum and pulmonary TNF-αon day 14 and day 28.Consistently,the lung tissue HYP content and serum and pulmonary PDGF-B levels on day 28 and day 56 were reduced

关 键 词：果王素 放射性肺损伤 肿瘤坏死因子α(TNF-α) 血小板源性生长因子B(PDGF-B) 羟脯氨酸 放射性肺炎 

分 类 号：R563[医药卫生—呼吸系统] R563.1[医药卫生—内科学] R392.12[医药卫生—临床医学] R392-33