核酸基质辅助激光解吸电离飞行时间质谱技术在结核病和非结核分枝杆菌病诊断中的临床应用专家共识  

Expert consensus on the clinical application of nucleic acid MALDI-TOF MS technique in the diagnosis of tuberculosis and non-tuberculosis mycobacteriosis

作　　者：中国人民解放军总医院第八医学中心结核病医学部 《中国防痨杂志》编辑委员会 中国医疗保健国际交流促进会结核病防治分会 梁建琴 吴雪琼 安慧茹 Senior Department of Tuberculosis,the 8th Medical Center of Chinese PLA General Hospital;Editorial Board of Chinese Journal of Antituberculosis;Tuberculosis Control Branch of China Intrnational Exchange and Promotive Association for Medical and Health Care;Liang Jianqin;Wu Xueqiong;An Huiru

机构地区：[1]不详 [2]中国人民解放军总医院第八医学中心

出　　处：《中国防痨杂志》2023年第6期543-558,共16页Chinese Journal of Antituberculosis

摘　　要：核酸基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI-TOF MS)技术在结核病与非结核分枝杆菌病病原学和耐药性诊断中的应用越来越多,为早期诊断、鉴别诊断和耐药鉴定提供了快速、准确的依据。然而,在实际应用中,临床医生对标本的选择、留取、送检时机及注意事项、报告结果的解读等的理解和掌握参差不齐,尚需规范化。本共识总结了核酸MALDI-TOF MS检测技术应用于结核病和非结核分枝杆菌病诊断的临床适应证和标本采集注意事项,介绍了如何正确解读核酸MALDI-TOF MS技术鉴定分枝杆菌菌种和耐药性的报告结果,以进一步规范核酸MALDI-TOF MS技术在结核病和非结核分枝杆菌病诊断中的临床应用,提高临床诊断水平,指导临床开展早期精准有效的治疗。Nucleic acid matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS)technique has been increasingly applied in the etiology and drug resistance diagnosis of tuberculosis and non-tuberculous mycobacteriosis,providing a rapid and accurate basis for early diagnosis,differential diagnosis,and drug resistance identification.However,it needs to be normalized that the clinician’s understanding and grasp of sample selection,retention,submitting time and precautions,and interpretation of report results.This consensus summarizes the clinical indications and specimen collection precautions of nucleic acid MALDI-TOF MS detection technique for the diagnosis of tuberculosis and non-tuberculous mycobacteriosis,and introduces how to correctly interpret the reported results of nucleic acid MALDI-TOF MS technique used to mycobacterial species and drug resistance.It has important significance to further standardize the clinical application of nucleic acid MALDI-TOF MS technique in the diagnosis of tuberculosis and non-tuberculous mycobacteriosis,improve the level of clinical diagnosis,and guide the early accurate and effective clinical treatment.

关 键 词：核酸类 质谱分析法 结核 分枝杆菌感染 总结性报告(主题) 

分 类 号：R52[医药卫生—内科学] R81[医药卫生—临床医学]