Cu^(2+)和阿维菌素及其复合胁迫对亚洲玉米螟生长发育和繁殖行为的影响  

Effects of Cu^(2+)and avermectin and their combined stress on the growth and development and reproductive behavior of Ostrinia furnacalis(Lepidoptera:Pyralidae)

作　　者：郭欣 徐凡舒 魏洪义[1] 陈丽慧 GUO Xin;XU Fan-Shu;WEI Hong-Yi;CHEN Li-Hui(School of Agronomy Sciences,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学农学院,南昌330045

出　　处：《昆虫学报》2023年第4期553-563,共11页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31760637,31640064);江西省教育厅科学技术研究项目(GJJ200439)。

摘　　要：【目的】探究农田重金属Cu^(2+)和农药阿维菌素(avermectin)及其复合胁迫对亚洲玉米螟Ostrinia furnacalis的生长发育和繁殖行为的影响。【方法】在人工饲料中分别和同时添加50 mg/kg Cu^(2+)和0.039 mg/L阿维菌素(对亚洲玉米螟3龄幼虫的LC_(10)浓度),得到含Cu^(2+)(Cu)、阿维菌素(Av)和同时含二者(Av+Cu)的3种处理的饲料,以正常人工饲料为空白对照组。实验室条件下用这4种饲料饲养亚洲玉米螟初孵幼虫,系统调查和记录其生长发育参数(幼虫历期、蛹历期、蛹重、成虫体重、生长速率、成虫羽化率和成虫寿命)、求偶行为参数(求偶率和平均求偶持续时间)、定向行为参数(起飞率、定向率、平均反应时间、飞过半程率和降落至信息素源率)以及繁殖参数(单雌产卵量和卵孵化率)。【结果】Cu和Av及其复合胁迫(Av+Cu)对亚洲玉米螟的生长发育和繁殖均产生一定影响。一方面,Cu单独处理后亚洲玉米螟的雌雄虫胚后发育期(幼虫历期+蛹历期)较空白对照组显著缩短;而Av单独处理后,亚洲玉米螟雌雄虫胚后发育期则分别显著延长至38.31和38.95 d,较空白对照组的分别长67.07%和67.38%。Cu单独处理后,亚洲玉米螟雌蛹、雄蛹、雌成虫和雄成虫体重较空白对照组均显著下降,分别比空白对照组的减轻13.16%,6.45%,7.13%和4.60%;Av单独处理后显著抑制亚洲玉米螟雌蛹、雄蛹和雌成虫体重,分别比空白对照组的减轻了26.50%,8.96%和12.46%,而雄成虫体重比空白对照组的显著增高6.05%。同时Cu处理对雌成虫的求偶行为产生一定影响,Cu处理后各个暗期最高求偶率和平均求偶持续时间均随暗期降低,而空白对照组的最高求偶率和平均求偶持续时间表现为随暗期先升高后降低的趋势;Av处理后促进雌成虫求偶行为:最高求偶率除暗期5(雌虫羽化后的第5个暗期)外在其他暗期均达到100%,平均求偶持续时间除暗期3(雌虫羽化�【Aim】To explore the effects of the heavy metal Cu^(2+)and the pesticide avermectin and their combined stress on the growth and development and reproductive behavior of the Asian corn borer,Ostrinia furnacalis in farmland.【Methods】We added 50 mg/kg of Cu^(2+)and 0.039 mg/L of avermectin(LC_(10) concentration against the 3rd instar larvae of O.furnacalis)to the artificial diet,respectively,and simultaneously,and then got three kinds of treated diets containing Cu^(2+)(Cu),avermectin(Av)and both(Av+Cu),taking the normal artificial diet as the blank control group.We fed the neonate larvae of O.furnacalis with the four diets under laboratory conditions,and systematically investigated and recorded the parameters of growth and development(larval duration,pupal duration,pupal weight,adult weight,growth rate,adult emergence rate and adult longevity),calling behavior(calling rate and average calling duration),orientation behavior(take-off rate,orientation rate,average response time,flying halfway rate and landing to pheromone source rate),and reproduction(number of eggs laid per female and egg hatching rate).【Results】Cu,Av and their combined stress(Av+Cu)had certain effects on the growth and development and reproduction of O.furnacalis.On the one hand,the postembryonic developmental duration(larval plus pupal duration)of female and male O.furnacalis in Cu treatment alone was significantly shortened as compared to that in the blank control group.After Av treatment alone,the postembryonic developmental duration of female and male O.furnacalis was significantly prolonged to 38.31 and 38.95 d,respectively,being 67.07%and 67.38%longer than that of the blank control group,respectively.After Cu treatment alone,the body weight of female pupae,male pupae,female adults and male adults of O.furnacalis decreased significantly,being 13.16%,6.45%,7.13%and 4.60%lower than that of the blank control group,respectively.Then after Av treatment alone,the body weight of female pupae,male pupae and female adults of O.furnacalis decr

关 键 词：亚洲玉米螟 铜 阿维菌素 生长发育 求偶行为 定向行为 生殖力 卵孵化率 

分 类 号：Q968[生物学—昆虫学]