基于RhoA/ROCK通路探究复方中药对腹水综合征肉鸡肝纤维化的影响  

Effects of compound Chinese medicine on hepatic fibrosis in ascites broilers based on RhoA/ROCK pathway

作　　者：王慧敏 崔亮 韩宇峰 张剑 王珂瑶 邓瑞强[1] 康杰[1] 郭东清 段智变[1] WANG Huimin;CUI Liang;HAN Yufeng;ZHANG Jian;WANG Keyao;DENG Ruiqiang;KANG Jie;GUO Dongqing;DUAN Zhibian(School of Veterinary Medicine,Shanxi Agricultural University,Taigu Shanxi 030801,China)

机构地区：[1]山西农业大学动物医学学院,山西太谷030801

出　　处：《东北农业大学学报》2023年第5期47-54,共8页Journal of Northeast Agricultural University

基　　金：山西省自然科学基金项目(201901D111235)。

摘　　要：为探讨复方中药水提液通过RhoA/ROCK通路对肉鸡腹水综合征(AS)肝纤维化的影响作用,将100羽7日龄罗斯肉鸡,随机平均分成空白组(K组),AS模型组(M组),复方中药水提液高、低剂量组(G、D组)以及L-精氨酸组(L-Arg组)5组。观察整体状态并记录15、25、35日龄肉鸡体重及35日龄腹水心脏指数(AHI);于35日龄剖检,取肝脏部分用于Masson染色观察胶原纤维变化,部分用于实时荧光定量PCR检测MYPT1 mRNA相对表达量和ELISA检测RhoA、ROCK、MLCP、P-MLC蛋白含量。与K组相比,M组AS肉鸡精神萎靡,腹部着地,腹腔内有大量腹水,Masson染色可观察到胶原纤维堆积,发生纤维化,15、25、35日龄M组肉鸡体重极显著降低(P<0.01),35日龄,M组肉鸡AHI和RhoA、ROCK、P-MLC蛋白含量极显著升高(P<0.01),MYPT1 mRNA表达显著升高(P<0.05),(Myosin light chain phosphatase,MLCP)蛋白活性极显著降低(P<0.01)。与M组相比,G、D组和L-Arg组肉鸡以上症状及各指标均有不同程度减轻,且G组作用效果明显。复方中药水提液和L-Arg可通过RhoA/ROCK通路,抑制肝纤维化,有效防治AS。In order to investigate the effect of water extract of compound Chinese through RhoA/ROCK pathway on hepatic fibrosis of broiler ascites syndrome(AS).The 1007-day-old Ross broilers were randomly divided into blank group(K group),AS model group(M group),compound Chinese medicine liquid extraction high and low dose group(G and D groups)and L-arginine group(L-Arg group)five groups.The overall state was observed and the weights of 15,25,and 35-day-old broilers and the asccites heart index(AHI)of 35-day-old were recorded.At the age of 35 days,the part of liver was used for Masson staining to observe collagen fiber changes,and part was used for real-time fluorescence quantitative PCR to detect the relative expressions of MYPT1 mRNA and ELISA to detect the contents of RhoA,ROCK,MLCP and p-MLC proteins.Compared with K group,AS broilers in M group were dispirited,landed on the abdomen and had a lot of ascites in the abdominal cavity.Masson staining showed a large accumulation of collagen fibers.The body weights of broilers in M group were significantly reduced at the age of 15,25 and 35 days(P<0.01).At the age of 35 days,the contents of AHI,RhoA,ROCK and P-MLC proteins in M group were significantly increased(P<0.01),and the expression of MYPT1 mRNA was significantly increased(P<0.05).The activity of MLCP protein decreased significantly(P<0.01).Compared with M group,the above symptoms and indexes of broilers in G,D and L-Arg groups were improved in varying degrees,and the effect of G group was the most significant.The phosphorylation of MLC mediated by RhoA/ROCK pathway was involved in the pathogenesis of AS.Compound traditional Chinese medicine water extract and L-Arg could effectively prevent and treat AS,and the mechanism was closely related to the inhibition of liver fibrosis by RhoA/ROCK pathway.

关 键 词：肉鸡腹水综合征 肝纤维化 RhoA/ROCK 复方中药水提液 L-ARG 

分 类 号：S858.31[农业科学—临床兽医学]