低剂量电离辐射对HBE细胞氧化应激及损伤修复影响  

Effects of low-dose radiation on oxidative stress and damage repair in HBE cells

作　　者：周林倩 黄伟旭[2] 蔡丽娜 柯伟奕 张灵钰 蔡雅诗 张素芬[2] 杨萍 邹剑明[2] 陈慧峰 ZHOU Linqian;HUANG Weixu;CAI Lina;KE Weiyi;ZHANG Lingyu;CAI Yashi;ZHANG Sufen;YANG Ping;ZOU Jianming;CHEN Huifeng(School of Public Health,Guangzhou Medical University,Guangzhou 511436 China;Guangdong Key Laboratory of Occupational Disease Prevention and Control,Guangdong Province Hospital for Occupational Disease Prevention and Treatment,Guangzhou 510300 China;School of Public Health,Guangdong Pharmaceutical University,Guangzhou 510006 China;School of Public Health,Southern Medical University,Guangzhou 510515 China)

机构地区：[1]广州医科大学公共卫生学院,广东广州511436 [2]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300 [3]广东药科大学公共卫生学院,广东广州510006 [4]南方医科大学公共卫生学院,广东广州510515

出　　处：《中国辐射卫生》2023年第2期150-155,共6页Chinese Journal of Radiological Health

基　　金：国家自然科学基金(81302387);广东省职业病防治重点实验室(2017B030314152);广东省基础与应用基础研究基金(2019A1515011969、2022A1515012421、2023A1515010414);广州市科技计划项目(202002030031);广东省医学科学技术研究基金(B2021386)。

摘　　要：目的 探讨低剂量电离辐射(LDIR)对HBE细胞氧化应激及损伤修复的影响。方法 将HBE细胞分为0、50、100、200 mGy组,采用X射线照射后分别培养24 h和48 h,检测细胞存活率、谷胱甘肽(GSH)、丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHdG)以及DNA损伤修复相关基因PPP2R2D和TP53基因转录水平。结果 与对照组相比,辐照后24 h,各剂量组细胞存活率差异无统计学意义(P> 0.05);各剂量组MDA水平均显著升高,GSH水平呈剂量依赖性降低,8-OHdG水平呈剂量依赖性升高,PPP2R2D基因mRNA水平均显著升高,差异具有统计学意义(P <0.05);50 mGy组TP53基因mRNA表达水平显著升高,差异具有统计学意义(P <0.05)。与对照组相比,辐照后48 h,50 mGy组细胞存活率达到最高,MDA水平显著降低,8-OHdG水平显著降低,PPP2R2D基因和TP53基因m RNA表达水平均显著升高,差异具有统计学意义(P <0.05);100 mGy组细胞GSH水平显著升高,差异具有统计学意义(P <0.05)。结论 LDIR特别是50 mGy的辐射可影响HBE细胞氧化-抗氧化水平以及DNA损伤修复相关基因转录水平差异表达。Objective To investigate the effects of lowdose ionizing radiation(LDIR)on oxidative stress and damage repair in human bronchial epithelial(HBE)cells.Methods HBE cells were divided into 0,50,100,and 200 mGy groups,and cultured for 24 and 48 h after X-ray irradiation,respectively.The cell viability,levels of glutathione(GSH),malondialdehyde(MDA),and 8-hydroxy-2’-deoxyguanosine(8-OHdG),and transcriptional levels of DNA damage repair genes PPP2R2D and TP53 were measured.Results At 24 h after irradiation,there was no significant difference in the cell viability between the dose groups and the control group(P>0.05);all dose groups had significantly increased MDA level,dosedependently decreased GSH level,dose-dependently increased 8-OHdG level,and significantly increased mRNA level of PPP2R2D gene(all P<0.05);the mRNA expression level of TP53 gene was significantly increased in the 50 mGy group(P<0.05).At 48 h after irradiation,there were the highest cell viability,significantly decreased MDA and 8-OHdG levels,and significantly increased mRNA expression levels of PPP2R2D and TP53 genes in the 50 mGy group compared with the control group(all P<0.05);the GSH level in the 100 mGy group was significantly increased(P<0.05).Conclusion LDIR,especially radiation at 50 mGy,can affect the oxidative-antioxidant level in HBE cells and the transcript-level differential expression of DNA damage repair genes.

关 键 词：低剂量电离辐射 氧化应激 DNA氧化损伤 DNA修复 

分 类 号：X591[环境科学与工程—环境工程]