臭椿酮对非小细胞肺癌顺铂耐药细胞A549/DDP的增敏作用  

Ailanthone Sensitizes Resistant Cell Line A549/DDP to Cisplatinin Non-small Cell Lung Cancer

作　　者：李祥伶 刘承一[2] 刘镭 陈龙 于胜利 许倩 LI Xiang-ling;LIU Cheng-yi;LIU Lei;CHEN Long;YU Sheng-li;XU Qian*(School of Basic Medicine,Chengde Medical University,Chengde,Hebei,067000,China;Affi liated Hospital of Chengde Medical University;Chengde Ecological environment inspection and monitoring Station)

机构地区：[1]承德医学院基础医学院,河北承德067000 [2]承德医学院附属医院 [3]承德市生态环境检验监测站

出　　处：《承德医学院学报》2023年第3期181-186,共6页Journal of Chengde Medical University

基　　金：国家自然科学基金项目(81703001);河北省重点研发计划项目(19277783D);河北省自然科学基金资助项目(H2021406021);河北省医学科学研究计划项目(20210247;20221335);承德医学院重大项目科研专项(KY2020005);政府资助临床医学优秀人才培养项目;河北省高校重点学科建设[冀教高(2013)4号]。

摘　　要：目的探讨臭椿酮(AIL)对非小细胞肺癌顺铂耐药细胞系A549/DDP的增敏作用。方法采用MTT检测AIL对A549和A549/DDP细胞活力的影响;Chou-Talalay中效法分析臭椿酮与顺铂的联合用药指数(CI);流式细胞术检测细胞周期及凋亡,Western blotting法检测凋亡相关蛋白表达水平。结果AIL(0.6μmol/L)与DDP(50μg/mL)联合用药有协同作用;细胞周期阻滞在G1期,同时细胞凋亡率升高;Caspase-3和Bcl-2蛋白表达下降,Cleaved Caspase3、Bax、CDK4和CyclinD1的蛋白表达升高。结论AIL可增强A549/DDP细胞对DDP的敏感性,协同DDP抑制A549/DDP细胞的生长,促进其凋亡。Objective To investigate the sensitization effect of Ailanthone(AIL)on cisplatin-resistant A549/DDP cells of non-small cell lung cancer.Methods The effects of AIL on the viability of A549 and A549/DDP cells were detected by MTT.Analysis of the combined drug use index(CI)of Ailanone and cisplatin using Chou-Talalay.Cell cycle and apoptosis were detected by fl ow cytometry,and the expression of apoptosis-related proteins were detected by Western blotting.Results AIL(0.6μmol/L)and DDP(50μg/mL)had synergistic effect.Cell cycle arrest and apoptosis rate increased in G1 phase.The protein expression of caspase-3 and bcl-2 decreased,but the protein expression of Cleaved Caspase3,Bax,CDK4 and CyclinD1 increased.Conclusion AIL enhanced the sensitivity of DDP to A549/DDP,and increased the growth inhibition and apoptosis of DDP on A549/DDP.

关 键 词：臭椿酮 顺铂 非小细胞肺癌 A549/DDP细胞 增敏 

分 类 号：R285[医药卫生—中药学]