基于网络药理学探究虎杖治疗糖尿病心肌病的作用机制  

Mechanism of Polygonum cuspidatum in treating diabetic cardiomyopathy based on network pharmacology

作　　者：陈怡晴 周蕾[1] CHEN Yiqing;ZHOU Lei(Department of Cardiology,The First Affliated Hospital of Nanjing Medical University,Nanjing 210029,Jiangsu,China)

机构地区：[1]南京医科大学第一附属医院心内科,江苏南京210029

出　　处：《上海大学学报（自然科学版）》2023年第2期244-252,共9页Journal of Shanghai University:Natural Science Edition

基　　金：国家自然科学基金面上资助项目(81970723)。

摘　　要：采用网络药理学的方法探究虎杖(Polygonum cuspidatum)治疗糖尿病心肌病(diabetic cardiomyopathy, DCM)的关键靶点和潜在作用机制.通过检索中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP)和查阅文献,获得虎杖的主要活性成分并预测其潜在的作用靶点.利用GeneCards和在线人类孟德尔遗传(online Mendelian inheritance in man, OMIM)数据库获得DCM的作用靶点,并绘制Venn图获得二者的交集靶点.使用String数据库和CytoScape软件构建虎杖-有效成分-交集靶点网络和蛋白相互作用(protein-protein interaction, PPI)网络.通过注释、可视化和集成发现数据库(the database for annotation, visualization and integrated discovery, DAVID)在线分析工具进行基因本体论(gene ontology, GO)富集分析和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes, KEGG)富集分析,共筛选出15个虎杖有效活性成分以及214个治疗DCM的潜在作用靶点,主要涉及脂质和动脉粥样硬化通路、糖尿病并发症中的AGE-RAGE信号通路、IL-17信号通路、HIF-1信号通路、胰岛素抵抗通路、PI3K-Akt信号通路及凋亡通路等.这说明虎杖通过多成分、多靶点、多通路来发挥治疗DCM的作用.To explore the key target and potential mechanism of Polygonum cuspidatum in the treatment of diabetic cardiomyopathy(DCM)by network pharmacology,the main active components of Polygonum cuspidatum were obtained by retrieval of traditional Chi-nese medicine systems pharmacology database and analysis platform(TCMSP)combined with literature review,and the potential targets were predicted.The targets of DCM were obtained by GeneCards and online Mendelian inheritance in man(OMIM)database and the intersection targets were obtained by drawing Venn diagram.The String database and CytoScape software were used to construct the network of Polygonum cuspidatum-active constituents-intersection target and protein-protein interaction(PPI)network.The target gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)en-richment analysis were conducted using the database for annotation,visualization and integrated discovery(DAVID).There were 15 active components and 214 potential targets of Polygonum cuspidatum in treatment of DCM,mainly involving lipid and atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,IL-17 signaling pathway,HIF-1 signaling pathway,insulin resistance,PI3K-Akt signaling pathway and apoptosis.These results demonstrate that Polygonum cuspidatum plays a key role in the treatment of DCM through multiple active components,multiple targets and multiple pathways.

关 键 词：虎杖 糖尿病心肌病 网络药理学 靶点 作用机制 

分 类 号：R541[医药卫生—心血管疾病]