Comparison of the conventional tube and erythrocyte-magnetized technology in titration of red blood cell alloantibodies  

作　　者：Xue-Hua He Hong Yan Chun-Yan Wang Xue-Yun Duan Jia-Jia Qiao Xiao-Jun Guo Hong-Bin Zhao Dong Ren Jian-She Li Qiang Zhang 

机构地区：[1]Department of Blood Transfusion,Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences,Tongji Shanxi Hospital,Third Hospital of Shanxi Medical University,Taiyuan 030032,Shanxi Province,China [2]Department of Blood Transfusion,The Second Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi Province,China [3]Department of Blood Transfusion,Shanxi Cancer Hospital,Taiyuan 030013,Shanxi Province,China [4]Department of Blood Transfusion,Shanxi Cardiovascular Hospital,Taiyuan 030024,Shanxi Province,China [5]Department of Clinical Laboratory,Taiyuan Blood Center,Institute of Blood Transfusion Technology,Taiyuan 030024,Shanxi Province,China

出　　处：《World Journal of Biological Chemistry》2023年第3期62-71,共10页世界生物化学杂志（英文版）（电子版）

基　　金：Supported by Project of Shanxi Provincial Health Commission,No.2021144.

摘　　要：BACKGROUND Erythrocyte alloantibodies are mainly produced after immune stimulation,such as blood transfusion,pregnancy,and transplantation,and are the leading causes of severe hemolytic transfusion reactions and difficulty in blood grouping and matching.Therefore,antibody screening is critical to prevent and improve red cell alloantibodies.Routine tube assay is the primary detection method of antibody screening.Recently,erythrocyte-magnetized technology(EMT)has been increasingly used in clinical practice.This study intends to probe the application and efficacy of the conventional tube and EMT in red blood cell alloantibody titration to provide a reference for clinical blood transfusion.AIM To investigate the application value of conventional tube and EMT in red blood cell alloantibody titration and enhance the safety of blood transfusion practice.METHODS A total of 1298 blood samples were harvested from blood donors at the Department of Blood Transfusion of our hospital from March 2021 to December 2022.A 5 mL blood sample was collected in tubing,which was then cut,and the whole blood was put into a test tube for centrifugation to separate the serum.Different red blood cell blood group antibody titers were simultaneously detected using the tube polybrene test,tube antiglobulin test(AGT),and EMT screening irregular antibody methods to determine the best test method.RESULTS Simultaneous detection was performed through the tube polybrene test,tube AGT and EMT screening irregular antibodies.It was discovered that the EMT screening irregular antibody method could detect all immunoglobulin G(IgG)and immunoglobulin M(IgM)irregular antibodies,and the results of manual tube AGT were satisfactory,but the operation time was lengthy,and the equipment had a large footprint.The EMT screening irregular antibody assay was also conducted to determine its activity against type O Rh(D)red blood cells,and the outcomes were satisfactory.Furthermore,compared to the conventional tube method,the EMT screening irregular antibody method wa

关 键 词：Erythrocyte-magnetized technology Conventional tube Red blood cell alloantibodies Transfusion reactions Application 

分 类 号：R446.61[医药卫生—诊断学]