普通野生稻内生细菌的分离鉴定及其对多年生稻的促生效果  

Screening and identification of endophytic bacteria from Oryza rufipogon and their effect on perennial rice growth

作　　者：马永海 田青霖 龚禹瑞 薛治峰 张廷萍 李丽萍[1] 杨红敏 黄立钰[1] 胡凤益[1] 秦世雯 MA Yong-hai;TIAN Qing-lin;GONG Yu-rui;XUE Zhi-feng;ZHANG Ting-ping;LI Li-ping;YANG Hong-min;HUANG Li-yu;HU Feng-yi;QIN Shi-wen(Institute of Resource Plants/Key Laboratory of Biology and Germplasm Innovation of Perennial Rice from Ministry of Agriculture and Rural Affairs,School of Agriculture,Yunnan University,Kunming 650091,Yunnan,China)

机构地区：[1]云南大学资源植物研究院/农业农村部多年生稻生物学与种质创新重点实验室,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2023年第3期768-778,共11页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省基础研究计划重点项目(202101AS070001);云南省基础研究计划面上项目(202101AT070021).

摘　　要：普通野生稻(Oryza rufipogon)是具有丰富的生境、遗传和微生物多样性的野生稻资源,挖掘其内生细菌资源可为普通野生稻微生态研究和生物菌肥开发提供菌种资源.研究首次从云南元江普通野生稻的根、茎和叶3种组织分离获得了111株内生细菌,其中82株具有溶磷能力,23株具有固氮作用,50株可产铁载体,18株可产吲哚乙酸(indole-3-acetic acid,IAA).分离自茎部的枯草芽孢杆菌(Bacillus subtilis)ORS2-5菌株、路德维希肠杆菌(Enterobacter ludwigii)ORS1-2菌株和分离自叶部的水稻科萨克氏菌(Kosakonia oryzae)ORL3-1具有较高的IAA分泌量,分别为11.69、22.81 mg/L和24.62 mg/L.3个菌株对多年生稻具有显著促生效果,可以不同程度地提高多年生稻幼苗的根长、鲜重、叶绿素和氮素质量比.研究结果将为多年生稻生态菌肥开发提供菌种资源,助力多年生稻绿色轻简化的生产模式.Oryza rufipogon that possess abundant habitat,genetic and microbial diversity is a valuable wild rice resource.The collection of endophytic bacterial strain resources from O.rufipogon could be used for micro-ecosystem research and application in agricultural fertilizer production.In this study,a total of 111 endophytic bacterial strains were isolated from O.rufipogon in Yuanjiang,Yunnan Province,including 82 phosphorus solubilizing bacteria,23 nitrogen-fixing bacteria,50 siderophore-producing bacteria and 18 indole-3-acetic acid(IAA)-producing bacteria strains.Strains of Bacillus subtilis ORS2-5 and Enterobacter ludwigii ORS1-2 were isolated from stem tissues,and Kosakonia oryzae ORL3-1 was isolated from leaf tissues,showing the highest IAA productivity with yields of 11.69,22.81 mg/L and 24.62 mg/L.The greenhouse experiment showed that these strains significantly promoted perennial rice growth.Our study indicated that endophytic bacterial strains isolated from O.rufipogon have great potential to be developed as microbial fertilizers for perennial rice,which will meet the simplified cultivation model of perennial rice.

关 键 词：普通野生稻 内生细菌 促进作用 微生物菌肥 多年生稻 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]