MEBT/MEBO对慢性难愈合创面组织内Nrf2/HO-1/NQO1信号通路的影响  

Effect of MEBT/MEBO on the Nrf2/HO⁃1/NQO1 Signaling Pathway in Tissues of Chronical Non⁃healing Wounds

作　　者：岑丽君 贺佐分 田馨如 郭文闻 李文武 葛星月 杨雅量 唐乾利[3] CEN Lijun;HE Zuofen;TIAN Xinru;GUO Wenwen;LI Wenwu;GE Xingyue;YANG Yaliang;TANG Qianli(Department of Blood Transfusion,The Affiliated Hospital of Youjiang Medical University for Nationalities,Baise,Guangxi 533000,China;Graduate School,Youjiang Medical University for Nationalities,Baise,Guangxi 533000,China;West Guangxi Key Laboratory for the Prevention and Treatment of High Incidence Diseases,Baise,Guangxi 533000,China)

机构地区：[1]右江民族医学院附属医院输血科,广西百色533000 [2]右江民族医学院研究生学院,广西百色533000 [3]右江民族医学院桂西高发病防治重点实验室,广西百色533000

出　　处：《中国烧伤创疡杂志》2023年第3期169-177,共9页The Chinese Journal of Burns Wounds & Surface Ulcers

基　　金：国家自然科学基金面上项目(81774327);广西医学高层次领军人才培养“139”计划资助项目(桂卫科教发[2018]22号)。

摘　　要：目的探讨皮肤再生医疗技术(MEBT/MEBO)对大鼠慢性难愈合创面组织内核转录因子红系2相关因子2(Nrf2)/血红素氧合酶1(HO⁃1)/醌氧化还原酶1(NQO1)信号通路的影响。方法选取90只SPF级Wistar雄性大鼠适应性饲养1周后,采用随机数表法将其随机分为空白组、对照组、模型组、MEBO组和贝复新组,每组18只。其中空白组大鼠只做备皮处理,对照组大鼠建立急性创面模型,模型组、MEBO组和贝复新组大鼠建立慢性难愈合创面模型。模型建立后,空白组大鼠备皮处皮肤及对照组、模型组大鼠创面予以生理盐水纱布换药处理,MEBO组大鼠创面予以湿润烧伤膏(MEBO)药纱换药处理,贝复新组大鼠创面予以重组牛碱性成纤维细胞生长因子凝胶药纱换药处理,对比观察各组大鼠创面愈合率、组织病理学变化情况以及皮肤/创面组织内丙二醛(MDA)、超氧化物歧化酶(SOD)及Nrf2、HO⁃1、NQO1蛋白与HO⁃1、NQO1 mRNA表达水平。结果干预第3天,各组大鼠创面愈合率尤以对照组最高、贝复新组次之(P均<0.05);干预第7、14天,各组大鼠创面愈合率尤以MEBO组最高、贝复新组次之(P均<0.05)。HE染色结果显示,干预第3、7、14天,对照组、MEBO组和贝复新组大鼠创面组织内坏死细胞及炎性细胞逐渐减少,成纤维细胞与毛细血管逐渐增多,而模型组大鼠创面组织内坏死细胞和炎性细胞减少不明显;Masson染色结果显示,干预第3、7、14天,对照组、MEBO组和贝复新组大鼠创面组织内胶原纤维由细少且紊乱逐渐趋于粗大且平整,而模型组大鼠创面组织内胶原纤维虽逐渐增粗、增多,但排列仍较紊乱。干预第7天,对照组、MEBO组和贝复新组大鼠创面组织内MDA表达水平均明显低于模型组(P均<0.05);干预第3、7天,对照组、MEBO组和贝复新组大鼠创面组织内SOD表达水平均明显高于模型组(P均<0.05)。干预第3、7天,MEBO组和贝复新组大鼠创面组织内Nrf2Objective To investigate the effect of MEBT/MEBO on the nuclear factor⁃erythroid 2⁃related factor 2(Nrf2)/heme oxygenase⁃1(HO⁃1)/quinone oxidoreductase 1(NQO1)signaling pathway in tissues of rats with chronical non⁃healing wounds.Methods 90 Wistar male rats of SPF grade were selected,and divided,after one week of adaptive feeding,into five groups using the random number table:blank group(n=18),control group(n=18),model group(n=18),MEBO group(n=18)and rb⁃bFGF group(n=18).Only skin preparation was performed on the rats in the blank group,acute wound models were established on the rats in the control group,and chronic non⁃healing wound models were established on the rats respectively in the model group,MEBO group and rb⁃bFGF group.After the models were estab⁃lished,the prepared skin of rats in the blank group and the wounds of rats both in the control group and model group were treated with dressing change of normal saline gauze,while the wounds of rats in the MEBO group were treated with dressing change of MEBO gauze,and the wounds in the rb⁃bFGF group were treated with dressing change of recombinant bovine basic fibroblast growth factor(rb⁃bFGF)gel gauze.The wound healing rate,histopathological changes,and expression levels of malondialdehyde(MDA),superoxide dismutase(SOD),Nrf2,HO⁃1,and NQO1 proteins,as well as HO⁃1 mRNA and NQO1 mRNA in the skin/wound tissues of each group were compared among the groups.Results On day 3 of intervention,the rats in the control group presented the highest wound healing rate,followed by that in the rb⁃bFGF group(all P<0.05).On day 7 and 14 of intervention,the rats in the MEBO group presented the highest wound healing rate,followed by that in the rb⁃bFGF group(all P<0.05).HE staining showed that on day 3,7 and 14 of intervention,the number of necrotic cells and inflammatory cells gradually decreased,and the number of fibroblasts and capillaries gradually increased in the wound tissues of rats respectively in the control group,MEBO group,and rb⁃bFGF gro

关 键 词：慢性难愈合创面 皮肤再生医疗技术 核转录因子红系2相关因子2/血红素氧合酶1/醌氧化还原酶1信号通路 氧化应激 创面修复 

分 类 号：R641[医药卫生—外科学]