中药抗休克合剂对Ⅲ度烫伤大鼠早期肺损伤的影响机制研究  

Mechanism of Action of Chinese Medicine Anti⁃shock Mixture on Early Lung Injury of Rats withⅢ⁃degree Scalds

作　　者：孟银秋 宋均辉 赵杰 赵正阳 胡万萱 李兴照[1] 胡德林[1] 孙业祥[3] MENG Yinqiu;SONG Junhui;ZHAO Jie;ZHAO Zhengyang;HU Wanxuan;LI Xingzhao;HU Delin;SUN Yexiang(Department of Burns,,The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230022,China;Department of TCM,,The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230022,China;Department of Burns and Wound Repair,The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230022,China)

机构地区：[1]安徽医科大学第一附属医院烧伤科,安徽合肥230022 [2]安徽医科大学第一附属医院中医科,安徽合肥230022 [3]安徽医科大学第一附属医院烧伤与创面修复科,安徽合肥230022

出　　处：《中国烧伤创疡杂志》2023年第3期194-199,共6页The Chinese Journal of Burns Wounds & Surface Ulcers

基　　金：国家自然科学基金(81272092);安徽医科大学第一附属医院临床研究重点基金(LCYJ2021ZD005)。

摘　　要：目的研究探讨中药抗休克合剂对Ⅲ度烫伤大鼠早期肺损伤的影响机制。方法选取32只健康SD大鼠适应性饲养1周后,按照随机数表法将其随机分为假伤组、烫伤组、LR组、LR+中药组,每组8只,其中假伤组大鼠备皮后做假伤处理,烫伤组大鼠备皮后建立Ⅲ度烫伤模型,LR组大鼠备皮后建立Ⅲ度烫伤模型并予以乳酸钠林格注射液(LR)补液,LR+中药组大鼠备皮后建立Ⅲ度烫伤模型并予以LR补液联合中药抗休克合剂灌胃,对比观察各组大鼠血清炎症因子水平以及肺组织湿重/干重(W/D)比值、氧化应激水平与p38丝裂原活化蛋白激酶(p38MAPK)、胞外信号调节激酶1/2(ERK1/2)通路活化程度。结果烫伤后各组大鼠血清肿瘤坏死因子⁃α(TNF⁃α)、白细胞介素(IL)⁃10、IL⁃18、髓过氧化物酶(MPO)、丙二醛(MDA)水平以及W/D比值均明显升高(F=112.352、80.455、40.716、79.321、270.356、60.784,P均<0.001),超氧化物歧化酶(SOD)水平明显降低(F=157.977,P<0.001),p38MAPK与ERK1/2通路活化程度均明显增加(F=402.669、3764.000,P均<0.001),且LR+中药组大鼠血清TNF⁃α、IL⁃18、MPO、MDA水平以及p38MAPK与ERK1/2通路活化程度均明显低于烫伤组和LR组(LR+中药组比烫伤组:q=11.070、9.439、16.000、23.970、30.480、100.500,P均<0.001;LR+中药组比LR组:q=4.579、5.864、6.286、9.444、10.980、51.310,P=0.015、P=0.002、P=0.001、P<0.001、P<0.001、P<0.001),IL⁃10、SOD水平均明显高于烫伤组和LR组(LR+中药组比烫伤组:q=10.490、16.240,P均<0.001;LR+中药组比LR组:q=6.583、6.339,P<0.001、P=0.001)。结论中药抗休克合剂可通过降低机体炎症反应及氧化应激损伤程度、抑制p38MAPK和ERK1/2信号通路磷酸化而减轻Ⅲ度烫伤大鼠早期肺组织损伤。Objective To study the mechanism of action of Chinese medicine anti⁃shock mixture on early lung injury of rats withⅢ⁃degree scalds.Methods 32 healthy SD rats,after adaptive feeding for one week,were divided,using the random number table,into sham group(n=8),scald group(n=8),LR group(n=8)and LR+Chinese medicine group(n=8).After skin preparation,rats in sham group weren’t made injury,rats in scald group were madeⅢ⁃degree scald model,rats in LR group were madeⅢ⁃degree scald model and given fluid replacement with sodium lactate Ringer’s injection(LR),while rats in LR+Chinese medicine group were also madeⅢ⁃degree scald model and given fluid replacement with sodium lactate Ringer’s injection(LR)plus intragastric injection of Chinese medicine anti⁃shock mixture.The levels of serum inflammatory factors,lung wet weight/dry weight(W/D)ratio,oxidative stress level,and the activa⁃tion of p38 mitogen⁃activated protein kinase(p38MAPK)and extracellular signal⁃regulated kinase 1/2(ERK1/2)pathways were compared among the four groups.Results After being scalded,the levels of serum tumor necrosis factor⁃α(TNF⁃α),interleukin⁃10(IL⁃10),interleukin⁃18(IL⁃18),myeloperoxidase(MPO),malondialdehyde(MDA),and the W/D ratio of rats were all significantly elevated in the four groups(F=112.352,80.455,40.716,79.321,270.356 and 60.784,all P<0.001),while the level of superoxide dismutase(SOD)were obviously decreased(F=157.977,P<0.001),and the activation degree of p38MAPK and ERK1/2 pathways were markedly increased(F=402.669 and 3764.000,both P<0.001).Moreover,the levels of serum TNF⁃α,IL⁃18,MPO and MDA,and the activation degree of p38MAPK and ERK1/2 pathways were all significantly lower in the LR+Chinese medicine group compared with scald group and LR group(LR+Chinese medicine group vs.scald group:q=11.070,9.439,16.000,23.970,30.480 and 100.500,all P<0.001;LR+Chinese medicine group vs.LR group:q=4.579,5.864,6.286,9.444,10.980 and 51.310,P=0.015,P=0.002,P=0.001,P<0.001,P<0.001,P<0.001),and the lev

关 键 词：烧伤 烫伤 休克 液体复苏 肺损伤 中药抗休克合剂 作用机制 

分 类 号：R285.5[医药卫生—中药学]