超高效液相色谱-串联质谱法测定湿疹膏中67种激素  

Determination of 67 kinds of hormones in eczema cream by ultra-high performance liquid chromatography-tandem mass spectrometry

作　　者：潘春燕 姚誉阳[1] 孟元华[1] 吴林林 PAN Chunyan;YAO Yuyang;MENG Yuanhua;WU Linlin(Analysis Department of Wuxi Center for Disease Control and Prevention,Wuxi 214023,China)

机构地区：[1]无锡市疾病预防控制中心理化检验科,无锡214023

出　　处：《分析试验室》2023年第5期652-658,共7页Chinese Journal of Analysis Laboratory

基　　金：无锡市卫生健康委医学重点学科项目(LCZX2021006);“双百”卫生人才-后备拔尖人才-朱鹏飞项目(HB2020099)资助。

摘　　要：建立了湿疹膏中67种激素的超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法。样品先经饱和NaCl溶液分散,再经乙酸乙酯提取,QuEChERS净化后,UPLC-MS/MS检测,选择Waters AcQuityBEH C18色谱柱(1.7μm, 2.1 mm×100 mm),多反应监测模式扫描。67种激素在相应的浓度范围内,线性关系良好,相关系数均大于0.995,在3个不同浓度加标水平下,平均回收率为71.8%~115.9%,相对标准偏差(RSD)为1.7%~12.5%,定量限(LOQ)范围为0.15~1.00μg/kg,该方法能够满足湿疹膏中67种激素的检测需求。An ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was established for the determination of 67 kinds of hormones in eczema cream.Samples were first dispersed with saturated sodium chloride solution,then the analytes were extracted with ethyl acetate,purified by modified QuEChERS adsorbents,and detected by UPLC-MS/MS.The hormones were separated on Waters AcQuity BEH C18(1.7μm,2.1 mm×100 mm)column,and detected with scheduled multi-reaction monitoring(sMRM)in positive and negative mode.As a result,67 kinds of hormones had good linear relationships in the corresponding concentration ranges with the correlation coefficients greater than 0.995.The average recoveries of 67 kinds of hormones at three spiked levels ranged from 71.8%to 115.9%,with the relative standard deviations(RSDs)of 1.7%-12.5%.The limits of quantification(LOQs)were 0.15-1.00μg/kg.This established method can meet the actual testing needs of 67 kinds of hormones in eczema cream samples.

关 键 词：激素 超高效液相色谱-串联质谱 湿疹膏 QUECHERS 

分 类 号：O657.63[理学—分析化学]