一种检测二氧化硫衍生物的黄酮类荧光探针的合成及细胞成像应用  

Synthesis of a flavonoid fluorescent probe for the detection of sulfur dioxide derivatives and cell imaging

作　　者：张万强[1] 曹家宝 顾家瑜 李柯娇 ZHANG Wanqiang;CAO Jiabao;GU Jiayu;LI Kejiao(College of Science,Xuchang University,Xuchang 461000,China)

机构地区：[1]许昌学院数理学院,许昌461000

出　　处：《分析试验室》2023年第5期683-688,共6页Chinese Journal of Analysis Laboratory

摘　　要：将黄酮衍生物与3-喹啉盐偶联,设计合成了一种新型HSO_(3)^(-)荧光探针HMQC。在磷酸盐缓冲体系中,探针HMQC能够实现高选择性、高灵敏度(58 nmol/L)、快速(150 s)检测HSO_(3)^(-)。通过核磁氢谱、质谱及理论计算探讨了探针的响应机理,证实了HSO_(3)^(-)与探针的C=C双键进行加成反应,导致荧光增强。探针HMQC能够用于活细胞中HSO3-的荧光成像检测,是一种很有潜力的HSO_(3)^(-)检测工具。A novel fluorescent probe HMQC was synthesized for HSO_(3)^(-)-detection by coupling flavonoid derivatives with 3-quinoline salt.In PBS buffer solution,the probe showed high selectivity,good sensitivity(58 nmol/L)and rapid response(150 s)for the detection of HSO_(3)^(-)-.The possible sensing mechanism of the probe was discussed by nuclear magnetic hydrogen spectroscopy,mass spectrometry and theoretical calculation,indicating that the addition reaction between HSO_(3)^(-)and the C=C bond of the probe led to the fluorescence enhancement.The probe HMQC could be used for the detection of HSO_(3)^(-)in living cells,making it to be a promising tool for detecting HSO_(3)^(-).

关 键 词：亚硫酸氢根 3-羟基黄酮 荧光探针 细胞成像 

分 类 号：O657.3[理学—分析化学]