Wnt信号通路相关分子LGR5、CDK5和βcatenin在结直肠侧向发育型肿瘤中的表达及其临床意义  

􀆼Expression and Clinical Significance of Wnt Pathway Related Molecules LGR5,CDK5 andβ-catenin in Colorectal Laterally Spreading Tumor

作　　者：韦二丹 丘新泽 吴江妮 彭鹏[1] 李信[1] 邹军[1] 黄杰安[1] 刘诗权 WEI Erdan;QIU Xinze;WU Jiangni;PENG Peng;LI Xin;ZOU Jun;HUANG Jie′an;LIU Shiquan(Department of Gastroenterology,The Second Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi,530007,China)

机构地区：[1]广西医科大学第二附属医院消化内科,广西南宁530007

出　　处：《广西科学》2023年第2期375-382,共8页Guangxi Sciences

基　　金：国家自然科学基金项目(81460380);广西自然科学基金项目(2020GXNSFAA159056);广西医学高层次骨干人才培养“139”计划培养人选项目(G202003016);广西中医药适宜技术开发与推广项目(GZSY2156)资助。

摘　　要：为探讨Wnt信号通路相关分子富含亮氨酸重复序列G蛋白偶联受体5(Leucine-rich repeat-containing G protein-coupled Receptor 5,LGR5)、细胞周期蛋白依赖性激酶5(Cyclin-Dependent Kinase 5,CDK5)和β-连环蛋白(β-catenin)在结直肠侧向发育型肿瘤(Laterally Spreading Tumor,LST)中的表达及其临床意义,收集2017年1月-2021年11月于广西医科大学第二附属医院行内镜治疗的56例LST患者的临床资料和组织标本,另收集58例结直肠隆起型腺瘤(Protruded-type colorectal Adenoma,PA)、44例结直肠癌(Colorectal Cancer,CRC)和相应的正常组织及相应临床资料,采用免疫组织化学染色法测定组织中Wnt通路相关分子LGR5、CDK5和β-catenin在LST、PA、CRC和正常组织中的表达。结果表明:LST不同亚型在性别、年龄、病变部位及病理类型上的差异不具有统计学意义(P>0.05),在病变大小上的差异具有统计学意义(P<0.05)。LST与PA在年龄、病变部位及病理学类型和病变大小上的差异有统计学意义(P<0.05),但性别差异无统计学意义(P>0.05)。免疫组织化学染色法测定显示,LGR5、CDK5及β-catenin在正常组织、PA、LST和CRC中的表达逐渐增高,并且3种蛋白在LST组织的表达水平均高于正常组织和PA。Wnt信号通路相关分子LGR5、CDK5和β-catenin在正常组织、PA、LST和CRC中的阳性表达率逐渐增高,提示Wnt信号通路及其相关因子LGR5、CDK5和β-catenin可能在LST的发生、发展中发挥重要作用。In order to investigate the expression and the clinicopathological significance of Wnt signaling path-way related molecules Leucine-rich repeat-containing G protein-coupled Receptor 5(LGR5),Cyclin-Depend-ent Kinase 5(CDK5)andβ-catenin in colorectal Laterally Spreading Tumor(LST),a total of 56 LST tissues were collected from January 2017 to November 2021 in the Second Affiliated Hospital of Guangxi Medical University.In addition,58 cases of Protruded-type colorectal Adenoma(PA),44 cases of Colorectal Cancer(CRC)and corresponding normal tissues and corresponding clinical data were collected.The expression of Wnt signaling pathway related molecules LGR5,CDK5 andβ-catenin were detected by immunohistochemistry to explore the clinical significance among LST,PA,CRC and normal tissues.The results showed that the difference of LST subtypes in gender,age,lesion location and pathological type was not statistical significant(P>0.05),but the difference in tumor size was significant and had statistical significance(P<0.05).There was statistical significance in age,lesion location,pathological type and tumor size between LST and PA patients(P<0.05),but no statistical significance in gender(P>0.05).Immunohistochemical staining showed that the positive expression of LGR5,CDK5 andβ-catenin in normal mucosal tissues,PA,LST and CRC were gradually increased,and the expression levels of LGR5,CDK5 andβ-catenin in LST were higher than those in normal mucosal tissues and PA.The positive expression rates of Wnt signaling pathway related molecules LGR5,CDK5 andβ-catenin in normal tissues,PA,LST and CRC gradually increased,suggesting that Wnt signaling pathway and its related factors LGR5,CDK5 andβ-catenin may play an important role in the occur-rence and development of LST.

关 键 词：结直肠侧向发育型肿瘤 结直肠癌 G蛋白偶联受体5 细胞周期蛋白依赖性激酶5 β-连环蛋白 

分 类 号：R446.8[医药卫生—诊断学] R735.3[医药卫生—临床医学]