Glutathione S-transferase genetic polymorphisms and fluoride-induced reproductive toxicity in men with idiopathic infertility  

作　　者：Jun He Yi Mu Miao Liu Bang-Wei Che Wen-Jun Zhang Ke-Hang Chen Kai-Fa Tang 

机构地区：[1]Department of Urology,The Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China [2]Institute of Medical Science,Guizhou Medical University,Guiyang 550004,China

出　　处：《Asian Journal of Andrology》2023年第3期404-409,共6页亚洲男性学杂志（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(No.81660263)and the Science and Technology Fund Project of Guizhou Health Commission(No.gzwkj2021-211).

摘　　要：Male infertility caused by idiopathic oligoasthenospermia(OAT)is known as idiopathic male infertility.Glutathione S-transferase(GST)and fluoride may play important roles in idiopathic male infertility,but their effects are still unknown.Our study examined the relationship between GST polymorphisms and fluoride-induced toxicity in idiopathic male infertility and determined the underlying mechanism.Sperm,blood,and urine samples were collected from 560 males.Fluoride levels were measured by a highly selective electrode method,and GST genotypes were identified using polymerase chain reaction(PCR)and PCR-restriction fragment length polymorphism(PCR-RFLP).Semen parameters,DNA fragmentation index(DFI),mitochondrial membrane potential(MMP),and oxidative stress(OS)biomarkers were statistically assessed at the P<0.05 level.Compared with healthy fertile group,semen parameters,fluoride levels,OS biomarkers,sex hormone levels,and MMP and DFI levels were lower in the idiopathic male infertility group.For glutathione S-transferase M1(GSTM1[-])and glutathione S-transferase T1(GSTT1[-])or glutathione S-transferase P1(GSTP1)mutant genotypes,levels of semen fluoride,OS,MMP,and DFI were considerably higher,and the mean levels of sperm parameters and testosterone were statistically significant in GSTM1(+),GSTT1(+),and GSTP1 wild-type genotypes.Both semen and blood fluoride levels were associated with oxidative stress in idiopathic male infertility patients.Elevated fluoride in semen with the genotypes listed above was linked to reproductive quality in idiopathic male infertility patients.In conclusion,GST polymorphisms and fluorine may have an indicative relationship between reproductive quality and sex hormone levels,and OS participates in the development of idiopathic male infertility.

关 键 词：FLUORINE glutathione S-transferase idiopathic male infertility oxidative stress POLYMORPHISM 

分 类 号：R697[医药卫生—泌尿科学]