骨质疏松患者Th17/Treg细胞平衡状况及其与骨代谢指标的相关性  

Expression of Th17/Treg balance in patients with osteoporosis and its relationship with bone metabolism indexes

作　　者：付海宁[1] 丁晓明[2] 李新宇 高博 陆雪颖 梁冠文 FU Hai-ning;DING Xiao-ming;LI Xin-yu;GAO Bo;LU Xue-ying;LIANG Guan-wen(Laboratory Department,Baoji Maternal and Child Health Hospital,Baoji 721000,Shaanxi,CHINA;Department of Internal Medicine,Baoji Maternal and Child Health Hospital,Baoji 721000,Shaanxi,CHINA;Second Department of Orthopedics,Baoji People's Hospital,Baoji 721000,Shaanxi,CHINA)

机构地区：[1]宝鸡市妇幼保健院检验科,陕西宝鸡721000 [2]宝鸡市妇幼保健院内科,陕西宝鸡721000 [3]宝鸡市人民医院骨二科,陕西宝鸡721000

出　　处：《海南医学》2023年第11期1590-1593,共4页Hainan Medical Journal

摘　　要：目的研究骨质疏松患者辅助性T细胞17(Th17)、调节性T细胞(Treg)细胞平衡状况及其与骨代谢指标的相关性。方法回顾性分析2019年3月至2022年2月在宝鸡市妇幼保健院诊治的120例骨质疏松患者(骨质疏松组)的临床资料,另选择同期在我院体检的100例健康者作为对照组。比较两组受检者的Th17、Treg、骨代谢炎性细胞因子指标[包括肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-10(IL-10)、转化生长因子-β(TGF-β)]及骨代谢蛋白酶活性指标[包括基质金属蛋白酶-2(MMP-2)、Ⅰ型胶原吡啶交联终肽(ICTP)、Ⅰ型前胶原氨基端原肽(PINP)]水平,采用Spearman相关性分析Th17、Treg与骨代谢指标的相关性。结果骨质疏松组患者的Th17水平为(2.30±0.41)%,明显高于对照组的(1.43±0.35)%,Treg水平为(3.18±0.78)%,明显低于对照组的(4.47±0.63)%,差异均有统计学意义(P<0.05);骨质疏松组患者的TNF-α、IL-6水平分别为(7.63±1.05)ng/mL、(33.60±5.35)pg/mL,明显高于对照组的(3.36±0.47)ng/mL、(20.34±3.28)pg/mL,IL-10、TGF-β水平分别为(16.61±2.19)pg/mL、(21.39±6.11)pg/mL,明显低于对照组的(29.51±3.55)pg/mL、(40.47±10.35)pg/mL,差异均有统计学意义(P<0.05);骨质疏松组患者的MMP-2、ICTP水平分别为(912.36±109.82)ng/mL、(6.50±0.84)ng/mL,明显高于对照组的(648.59±75.28)ng/mL、(4.02±0.56)ng/mL,PINP水平为(0.85±0.13)ng/mL,明显低于对照组的(1.44±0.20)ng/mL,差异均有统计学意义(P<0.05);经Spearman分析结果显示,Th17与TNF-α、MMP-2、ICTP呈正相关(P<0.05),Treg与TNF-α、MMP-2、ICTP呈负相关(P<0.05),与TGF-β呈正相关(P<0.05)。结论骨质疏松患者中Th17水平提升、Treg表达水平降低,它们共同干扰骨代谢期间的炎性反应与蛋白酶活性。Objective To study the expression significance of helper T cell 17(Th17)and regulatory T cell(Treg)cell balance in patients with osteoporosis and their relationship with bone metabolism indexes.Methods The clinical data of 120 patients with osteoporosis(osteoporosis group)diagnosed and treated in Baoji Maternal and Child Health Hospital from March 2019 to February 2022 were retrospectively analyzed.Another 100 healthy patients who un-derwent physical examination in the hospital during the same period were selected as the control group.The Th17,Treg,bone metabolism inflammatory cytokine indexes[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleu-kin-10(IL-10),transforming growth factor-β(TGF-β)],and bone metabolism protease activity indexes[matrix metallo-proteinase-2(MMP-2),type I collagen pyridine cross-linking terminal peptide(ICTP),type I procollagen amino terminal propeptide(PINP)]levels in the two groups were compared.Spearman correlation was used to analyze the correlation be-tween Th17,Treg and bone metabolism indexes.Results The Th17 level in the osteoporosis group was(2.30±0.41)%,which was significantly higher than(1.43±0.35)%in the control group,and the Treg level was(3.18±0.78)%,which was significantly lower than(4.47±0.63)%in the control group,with statistically significant differences(P<0.05).The levels of TNF-αand IL-6 in the osteoporosis group were(7.63±1.05)ng/mL and(33.60±5.35)pg/mL,which were significant-ly higher than(3.36±0.47)ng/mL,(20.34±3.28)pg/mL in the control group,and IL-10 and TGF-βlevels were(16.61±2.19)pg/mL,(21.39±6.11)pg/mL,significantly lower than(29.51±3.55)pg/mL,(40.47±10.35)pg/mL in the control group,with statistically significant differences(P<0.05).The levels of MMP-2 and ICTP in osteoporosis group were(912.36±109.82)ng/mL and(6.50±0.84)ng/mL,significantly higher than(648.59±75.28)ng/mL,(4.02±0.56)ng/mL in the control group,and PINP level was(0.85±0.13)ng/mL,significantly lower than(1.44±0.20)ng/mL in the control group,with statistically signif

关 键 词：骨质疏松 辅助性T细胞17 调节性T细胞 骨代谢 相关性 

分 类 号：R681[医药卫生—骨科学]