实验性脊髓损伤后大脑血管可塑性的磁共振血管尺寸成像研究  

In vivo MR Vessel Size Imaging of Brain Vascular Plasticity After Experimental Spinal Cord Injury

作　　者：田雨 周臣[2,3] 张亚男 王鹏 张彩云[4] 宋天玮 钱俊超 TIAN Yu;ZHOU Chen;ZHANG Yanan;WANG Peng;ZHANG Caiyun;SONG Tianwei;QIAN Junchao(School of Basic Medical Sciences,Anhui Medical University,Hefei 230032,China;Hefei Cancer Hospital,Chinese Academy of Sciences,Hefei 230031,China;Anhui Province Key Laboratory of Medical Physics and Technology,Institute of Health and Medical Technology,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China;Binzhou Medical University,Yantai 264003,China)

机构地区：[1]安徽医科大学基础医学院,安徽合肥230032 [2]中国科学院合肥肿瘤医院,安徽合肥230031 [3]中国科学院合肥物质科学研究院,健康与医学技术研究所,医学物理与技术安徽省重点实验室,安徽合肥230031 [4]滨州医学院,山东烟台264003

出　　处：《波谱学杂志》2023年第2期158-168,共11页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目(U1932158,81871085,82271519);山东省自然科学基金肿瘤防治联合基金重点项目(ZR2019LZL018);安徽省自然科学基金杰出青年项目(2208085J10);中国科学院合肥大科学中心协同创新培育基金重要项目(2019HSC-CIP003);中国博士后科学基金项目(2019M652403);山东省博士后创新项目(202002048).

摘　　要：本文应用7 T高场磁共振血管尺寸成像技术研究了大鼠脊髓半切损伤后大脑血管的可塑性.通过感兴趣区域分析发现,损伤4周后,与损伤部位同侧锥体相比,对侧锥体区域的平均血管直径、微血管密度和血管尺寸指数显著增加,提示对侧皮质脊髓束白质区域血管生成或活化.该结果经由免疫荧光实验证实,损伤4周后,对侧锥体区域血小板内皮细胞粘附分子和胶质纤维酸性蛋白的染色强度也显著增加.以上结果表明磁共振血管尺寸成像技术可为脊髓损伤后大脑相关区域的血管生成提供有价值的信息,并有可能成为诊断脊髓损伤患者脑部血管病变的新工具.In vivo magnetic resonance vessel size imaging at 7 T high field was used to explore the changes in cerebral vascular plasticity after spinal cord hemisection injury in rats.Region of interest(ROI)analysis showed that four weeks after injury,mean vessel diameter(mVD),microvascular density(Density)and vessel size index(VSI)values were significantly increased in the contralateral side compared with the ipsilateral pyramid of the injury site,suggesting angiogenesis or vascular activation in the white matter region of the contralateral corticospinal tract(CST).Immunofluorescence results showed that the intensity of staining for platelet endothelial cell adhesion molecule 1(CD31)and glial fibrillary acidic protein(GFAP)also increased significantly in the contralateral pyramid four weeks after injury.These results suggested that magnetic resonance vessel size imaging could provide valuable information on neovascularization in brain after spinal cord injury and may be a novel tool to diagnose brain vascular pathologies in spinal cord injury patients.

关 键 词：大脑血管可塑性 血管尺寸成像 大鼠 脊髓损伤 高场磁共振成像 

分 类 号：R338[医药卫生—人体生理学] R445.2[医药卫生—基础医学] O482.53[理学—固体物理]