兰溪市120例HIV抗体筛查阳性样本确证结果分析  

Confirmatory testing of 120 positive HIV antibody screening samples in Lanxi City

作　　者：徐亚华[1] 吴斌[2] XU Yahua;WU Bin(Department of Microbiology,Lanxi Center for Disease Control and Prevention,Lanxi,Zhejiang 321100,China;Jinhua Center for Disease Control and Prevention,Jinhua,Zhejiang 321002,China)

机构地区：[1]兰溪市疾病预防控制中心微生物检验科,浙江兰溪321100 [2]金华市疾病预防控制中心,浙江金华321002

出　　处：《预防医学》2023年第6期538-540,共3页CHINA PREVENTIVE MEDICINE JOURNAL

摘　　要：目的分析浙江省兰溪市120例HIV抗体筛查阳性样本的确证结果,为完善艾滋病检测和防控工作提供依据。方法对2020—2022年兰溪市各HIV筛查实验室HIV抗体筛查阳性的120例样本进行蛋白免疫印迹试验;按照《全国艾滋病检测技术规范(2020年修订版)》和WS 293—2019《艾滋病和艾滋病病毒感染诊断标准》判定确证结果,并分析确证阳性率及带型分布。结果120例HIV抗体筛查阳性样本确证为HIV-1抗体阳性100例,阳性率为83.33%;HIV抗体阴性6例,占5.00%;HIV-1抗体不确定14例,占11.67%。HIV-1抗体阳性样本中,男性72例,女性28例,男女性别比为2.57∶1;年龄20~<60岁88例,占88.00%;gp160、gp120和p24带型出现频率较高,均为100.00%。HIV-1抗体不确定样本中,p24带型出现频率较高,为78.57%。HIV-1抗体不确定样本经随访检测确定6例(带型为gp160+p24)为HIV-1补充试验阳性,8例为HIV抗体阴性。结论兰溪市120例HIV抗体筛查阳性样本的HIV-1抗体阳性率为83.33%;gp160、gp120和p24带型出现频率较高;对HIV-1抗体不确定样本需加强随访,做到早发现、早干预。Objective To analyze the confirmatory test results of 120 positive HIV antibody screening samples in Lanxi City,Zhejiang Province,so as to provide insights into AIDS detection and control.Methods Western blotting confirmatory assay was performed on 120 positive HIV antibody samples from HIV screening laboratories in Lanxi City from 2020 to 2022.The confirmatory test results were determined according to the“National Technical Guidelines for AIDS Testing(2020 revision)”and“Diagnosis for HIV/AIDS”(WS 293-2019),and the positive rate of confirmatory test and band distribution were analyzed.Results Among 120 positive HIV antibody screening samples,there were 100 HIV-1 antibody positive samples(83.33%)as revealed by Western blotting confirmatory assay,6 HIV antibody negative samples(5.00%),and 14 HIV-1 indeterminate tests(11.67%).There were 72 men and 28 men tested positive for HIV-1 antibody,with a male to female ratio of 2.57∶1,and there were 88 patients at ages of 20 to 59 years(88.00%),with the highest frequency of gp160,gp120 and p24 bands(100.00%).The most common Western blotting band pattern was p24 among HIV-1 indeterminate tests(78.57%).Among all HIV-1 indeterminate Western blotting tests,6 cases were tested positive for HIV-1 antibody(gp160+p24)and 8 cases tested negative for HIV antibody during the follow-up period.Conclusions The prevalence of HIV-1 antibody was 83.33%in 120 positive HIV antibody screening samples in Lanxi City.The three most common Western blotting band patterns were gp160,gp120 and p24.The follow-up requires to be reinforced in HIV-1 indeterminate Western blotting tests for early identification and early intervention.

关 键 词：艾滋病 HIV抗体筛查 确证试验 

分 类 号：R512.91[医药卫生—内科学]