谷子KCS基因家族鉴定及其对干旱胁迫的响应  

Identification and Response to Drought Stress of KCS Gene Family in Foxtail Millet

作　　者：朱垠豪 李晨 葛星辰 朱倩倩 段明 韩渊怀[1,3] 马芳芳 ZHU Yinhao;LI Chen;GE Xingchen;ZHU Qianqian;DUAN Ming;HAN Yuanhuai;MA Fangfang(College of Agriculture,Shanxi Agricultural University,Taigu 030801,China;Experimental Teaching Center,Shanxi Agricultural University,Taigu 030801,China;Shanxi Key Laboratory of Minor Crop Germplasm Innovation and Molecular Breeding,Taigu 030801,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学实验教学中心,山西太谷030801 [3]杂粮种质创新与分子育种山西省重点实验室,山西太谷030801

出　　处：《山西农业科学》2023年第6期599-609,共11页Journal of Shanxi Agricultural Sciences

基　　金：山西省留学人员科技活动择优资助项目(20220015);山西省基础研究计划项目(2021032123395);谷子种业创新良种联合攻关项目(2022XCZX04-10)。

摘　　要：酮脂酰辅酶A合酶(β-ketoacyl-CoA synthase,KCS)是超长链脂肪酸合成过程中的限速酶,主要负责调控脂肪酸和蜡质的合成与积累。为了为谷子抗旱品种的选育提供新的线索,研究利用隐马尔可夫模型对谷子KSC基因家族进行鉴定,并利用生物信息学技术对谷子KCS基因家族进行系统分析与鉴定,并对其理化特征、进化关系、保守基序、基因结构、顺式调控元件、亚细胞定位、共线性进行分析和表达模式分析。结果表明,从谷子基因组中共鉴定到33个KCS基因;系统进化和共线性分析结果显示,谷子KCS基因与高粱、玉米进化关系更近;启动子区包含许多激素响应、逆境响应和生长发育相关元件,暗示KCS基因可能参与多个生物学过程;谷子KCS基因在穗部表达量最高,其次为叶片,早晚表达量均高于中午。此外,干旱胁迫下谷子Seita.9G470700和Seita.9G487500基因的表达量显著高于对照,表明这2个KCS基因在谷子干旱响应中发挥重要作用。Theβ-ketoacyl-CoA synthase(KCS)is the rate-limiting enzyme of very long chain fatty acids(VLCFAs)synthesis,which is mainly responsible for regulating the synthesis and accumulation of fatty acids and wax.In this study,to provide a new clue for breeding of foxtail millet resistant varieties,the KSC gene family were identified in foxtail millet using HMM model,the KCS gene family of millet was systematically analyzed and identified by bioinformatics technology and their physical and chemical characteristics,evolutionary relationships,conservative motifs,gene structures,cis-acting elements,subcellular localization,and collinearity analysis and expression pattern were conducted.The results showed that a total of 33 SiKCS genes were identified in foxtail millet genome.Phylogenetic and collinearity analysis showed that SiKCSs were more closely related to sorghum and maize.The promoter region contained many hormone responses,stress responses,growth and development-related elements,indicating that SiKCSs were probably involved in multiple biological processes.The expression level of SiKCSs was the highest in panicle,followed by leaf,and was higher in the morning and evening than that in the noon.Additionally,the expression level of Seita.9G470700 and Seita.9G487500 genes under drought stress were significantly higher than that of the control,suggesting these two SiKCSs genes played an important role in drought stress response.

关 键 词：谷子 β–酮脂酰辅酶A合酶 生物信息学分析 表达分析 干旱胁迫响应 

分 类 号：S515[农业科学—作物学]