交泰丸对复合刺激致失眠大鼠GFAP和S100B的影响  

Effect of Jiaotai Pill on GFAP and S100B in Rats with Insomnia Caused by Compound Stimulation

作　　者：郭昕子 曾雪爱 周春权 黄俊山 GUO Xinzi;ZENG Xueai;ZHOU Chunquan;HUANG Junshan(Fujian University of Traditional Chinese Medical,Fujian Fuzhou 350122,China;Fujian Academy of Chinese Medicine Sciences,Fujian Fuzhou 350003,China)

机构地区：[1]福建中医药大学中西医结合研究院,福建福州350122 [2]福建省中医药科学院,福建福州350003

出　　处：《中医药临床杂志》2023年第5期971-975,共5页Clinical Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81774285);福建省科技厅公益类科研院所专项(2021R1003009);福建省中医药科研项目(2021zyjc07);福建省中医睡眠医学重点实验室(闽科基[2015]7号)。

摘　　要：目的:观察交泰丸对多因素刺激+对氯苯丙氨酸(para-chlorophenylalanine,PCPA)复合刺激致失眠大鼠血清中胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、S100钙结合蛋白B(S100 calcium-binding protein B,S100B)含量与基底前脑内GFAP、S100B蛋白相对表达量的影响。方法:将28只SD大鼠随机分为正常组、模型组、交泰丸组、西药组,每组各7只。除正常组外,其余各组大鼠采用随机多因素刺激+腹腔注射PCPA建立眠模型,造模周期16d。第17天开始灌胃给药,交泰丸组给予交泰丸药液,西药组给予艾司唑仑药液,正常组和模型组给予蒸馏水,1次/d,共7d。第16天和第23天观察动物的一般状态,第16天至第23天期间每天记录体质量,末次给药后ELISA法检测大鼠血清GFAP和S100B含量,Western Blot法检测大鼠基底前脑GFAP、S100B蛋白相对表达量。结果:与正常组比较,模型组大鼠一般状态较差,体质量增加缓慢(P<0.01),血清中GFAP、S100B含量升高(P<0.05或P<0.01),基底前脑内GFAP、S100B蛋白相对表达量升高(P<0.01)。与模型组相比,交泰丸组和西药组一般状态较好,体质量增量较多(P<0.05或P<0.01),血清中GFAP、S100B含量降低(P<0.05或P<0.01),基底前脑内GFAP、S100B蛋白相对表达量降低(P<0.01)。结论:交泰丸可降低血清中GFAP、S100B含量和基底前脑内GFAP、S100B蛋白相对表达量,减轻大脑神经细胞的损伤,改善睡眠。Objective:To observe the effect of Jiaotai Pills on glial fibrillary acidic protein(GFAP) and S100 calcium binding in serum of rats with insomnia induced by multifactorial stimulation + para-chlorophenylalanine(PCPA) combined stimulation The effect of protein B(S100 calcium-binding protein B,S100B) content on the relative expression of GFAP and S100B protein in the basal forebrain.Method:28 SD rats were randomly divided into normal group,model group,Jiaotai Pill group and western medicine group,with 7 rats in each group.Except for the normal group,rats in other groups were randomly stimulated by multiple factors + intraperitoneal injection of PCPA to establish sleep models,and the modeling period was 16 days.On the 17th day,intragastric administration began.The Jiaotai pill group was given Jiaotai pill liquid,the western medicine group was given estazolam liquid,and the normal group and model group were given distilled water,once a day,for a total of 7 days.The general state of the animals was observed on the 16th and 23rd days,and the body weight was recorded every day from the 16th to the 23rd day.After the last administration,the ELISA method was used to detect the rat serum GFAP and S100B levels,and the Western Blot method was used to detect the rat basal forebrain Relative expression of GFAP and S100B proteins.Results:Compared with the normal group,the rats in the model group were in poor general condition,with slow weight gain(P<0.01),elevated serum GFAP and S100B levels(P<0.05,P<0.01),GFAP,S100B in the basal forebrain,The relative expression of S100B protein increased(P<0.01).Compared with the model group,the Jiaotai pill group and the western medicine group had better general state,more body weight gain(P<0.05,P<0.01),and lower serum GFAP and S100B contents(P<0.05,P<0.01),the relative expression of GFAP and S100B proteins in the basal forebrain decreased(P<0.01).Conclusion:Jiaotai pill can reduce the content of GFAP and S100B in serum and the relative expression of GFAP and S100B protein in basal forebrai

关 键 词：失眠 交泰丸 胶质纤维酸性蛋白 S100钙结合蛋白B 

分 类 号：R285.5[医药卫生—中药学]