Dietary host-associated Bacillus subtilis supplementation improves intestinal microbiota,health and disease resistance in Chinese perch(Siniperca chuatsi)  

作　　者：Zhehui Ji Chuanzhong Zhu Xinyang Zhu Sainan Ban Lijuan Yu Juan Tian Lixue Dong Hua Wen Xing Lu Ming Jiang 

机构地区：[1]Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan,China [2]Fujian Key Laboratory of Functional Aquafeed and Culture Environment Control,Fujian DBN-HY Aquatic Science and Technology Group Co.,Ltd,Zhao’an,China [3]Beijing DBN Technology Group Co.,Ltd,Beijing,China

出　　处：《Animal Nutrition》2023年第2期197-205,共9页动物营养（英文版）

基　　金：supported by the Fund of Fujian Key Laboratory of Functional Aquafeed and Culture Environment Control(FACE20200004)and China Agriculture Research System(CARS-46).

摘　　要：The purpose of this study was to evaluate the potential of a host-associated Bacillus subtilis 1-C-7 as a probiotic for Chinese perch(Siniperca chuatsi).Four test diets were formulated to contain graded levels of B.subtilis 1-C-7 at 0(CY),0.85×10^(8)(Y1),0.95×10^(9)(Y2)and 0.91×10^(10)(Y3)CFU/kg diet.The test fish with initial weight 30.0±1.2 g were fed the 4 test diets with 3 replicates in an indoor water-flow aquaculture system with 12 net cages(40 fish/cage)for 10 wk.At the conclusion of the feeding trial,the probiotic effects of B.subtilis on Chinese perch were analyzed based on growth performance,serum biochemical indices,histologic morphology of liver and gut,gut microbiota and the resistance to Aero-monas hydrophila.The results showed that the percentage of weight gain had no significant change in the Y1 and Y2 groups(P>0.05)but decreased in the Y3 group compared to that in the CY group(P<0.05).The fish in the Y3 group displayed the highest activity of serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)among these 4 groups(P<0.05).The fish in the CY group had the highest value of malondialdehyde in the liver(P<0.05)and showed severe nuclear migration and vacuolization of hepatocytes.The morphology indicated that all test fish had poor intestinal health.However,the fish in the Y1 group had a relatively normal intestinal histologic structure.The mid gut microbial diversity analysis showed that dietary B.subtilis supplementation increased the abundance of probiotics such as Tenericutes and Bacteroides,whereas it reduced the abundance of pernicious bacteria such as Proteobacteria,Actinobacteria,Thermophilia and Spirochaetes.The challenge test showed that dietary B.subtilis supplementation increased the resistance to A.hydrophila in Chinese perch.In conclusion,dietary supplementation of 0.85×10^(8)CFU/kg B.subtilis 1-C-7 could improve the intestinal microbiota,intestinal health and disease resistance in Chinese perch,but more or excessive supple-mentation could reduce growth performance

关 键 词：Bacillus subtilis Intestinal microbiota Intestinal health Disease resistance Chinese perch(Siniperca chuatsi) 

分 类 号：S965.211[农业科学—水产养殖]