刀鲚内皮素1基因SNP多态性及其与颌骨性状相关联分析  

SNP polymorphisms in Edn1 gene and their associated maxilla length traits in Coilia nasus

作　　者：高俊 周琳 聂志娟[1] 王耀辉 徐钢春[1] GAO Jun;ZHOU Lin;NIE Zhijuan;WANG Yaohui;XU Gangchun(Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs/Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214128,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Nantong Longyang Aquatic Products Co.,Ltd.,Nantong 226634,China)

机构地区：[1]中国水产科学研究院淡水渔业研究中心,农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡214081 [2]上海海洋大学水产与生命学院,上海201306 [3]南通龙洋水产有限公司,江苏南通226634

出　　处：《中国水产科学》2023年第3期259-267,共9页Journal of Fishery Sciences of China

基　　金：江苏省自然科学基金面上项目(BK20191145);江苏省种业振兴“揭榜挂帅”项目(JSGS[2021]134).

摘　　要：为探究刀鲚(Coilia nasus)内皮素1(endothelin 1,Edn1)基因对刀鲚上颌骨长度的影响,根据此前的基因组重测序结果以及刀鲚基因组数据库,通过PCR扩增得到刀鲚Edn1基因序列,并使用直接测序法筛选并分析单核苷酸多态性(single nucleotide polymorphism,SNP)位点,分析各位点的基因型,并计算各位点的基本遗传参数、基因型频率和基因频率,将筛选出的SNP位点与刀鲚上颌骨长度性状进行关联分析、连锁不平衡分析和双倍型分析。结果表明,在刀鲚Edn1基因中共挖掘出5个SNP位点,分别为T134C、A418C、G1322A、C1607T、A1636C。G1322A与A1636C两个位点之间处于连锁不平衡状态。关联分析结果显示,T134C位点和A418C位点与上颌骨长度性状之间存在显著相关性;双倍型分析结果显示,T134C位点和A418C位点组成的双倍型中,D3(杂合TC和纯合AA)的上颌骨长度显著长于D1(纯合TT和杂合AC)和D2(纯合TT和纯合CC)。此外,刀鲚开口高度/头长和上颌骨长/头长的皮尔逊相关系数r=0.41,属中等正相关。研究结果表明,T134C位点和A418C位点是刀鲚上颌骨长度的优势位点,双倍型D3为优势基因型。本研究可为刀鲚长颌骨分子标记辅助选育的候选标记提供科学依据。As the most intuitive phenotype,the length of the maxilla of Coilia nasus has always been considered as the main feature that distinguishes different ecological groups of C.nasus.Endothelin 1(Edn1)plays an essential role in jaw development.Based on the previous genome re-sequencing results and the C.nasus genome database,the Edn1 gene sequence was isolated using PCR amplification to investigate the effect of Edn1 gene on the maxilla length in C.nasus.The direct sequencing method was used to screen and analyze single nucleotide polymorphism(SNP)loci,analyze the genotype of each locus,and calculate the basic genetic parameters,genotype frequency,and gene frequency of each locus.Subsequently,association,linkage disequilibrium,and diplotype analyses were performed between these screened SNP loci and C.nasus maxillary length traits.The results showed that a total of five SNP loci were excavated in the Edn1 gene of C.nasus,including T134C,A418C,G1322A,C1607T,and A1636C.The two loci G1322A and A1636C are in linkage disequilibrium.Association analysis results showed that there was a significant correlation between T134C and A418C loci and maxilla length traits.Diplotype analysis showed that T134C and A418C loci formed diplotype D3(heterozygous TC and Homozygous AA),which had significantly longer maxilla lengths than D1(homozygous TT and heterozygous AC)and D2(homozygous TT and homozygous CC).In addition,the Pearson correlation coefficient r=0.41 for the mouth-opening height/head length and maxilla length/head length of C.nasus.These findings indicate that the T134C and A418C loci were the dominant loci for the maxilla length of C.nasus,and the diploid D3 was the dominant genotype.This study can provide a scientific foundation for the candidate markers for molecular marker-assisted selection of the C.nasus with long maxilla.

关 键 词：刀鲚 Edn1基因 性状关联分析 双倍型分析 连锁不平衡 

分 类 号：S917[农业科学—水产科学]