暗纹东方鲀渗透压调节相关基因响应盐度变化的表达特征  

Expression characteristics of genes related to osmotic pressure regulation in Takifugu obscurus during salinity acclimation

作　　者：刘鑫[1,2] 梁夏颖 王超宇 高阳 姚云龙[1,2] 赵哲 史燕 LIU Xin;LIANG Xiaying;WANG Chaoyu;GAO Yang;YAO Yunlong;ZHAO Zhe;SHI Yan(Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization,Hohai University,Nanjing 210024,China;Department of Marine Biology,College of Oceanography,Hohai University,Nanjing 210024,China)

机构地区：[1]河海大学,江苏省海洋生物资源可持续利用工程研究中心,江苏南京210024 [2]河海大学海洋学院海洋生物系,江苏南京210024

出　　处：《中国水产科学》2023年第3期297-308,共12页Journal of Fishery Sciences of China

基　　金：国家重点研发计划项目(2018YFD0900200);江苏省种业振兴揭榜挂帅项目(JBGS[2021]133);国家自然科学基金项目(32002424).

摘　　要：为了探索暗纹东方鲀(Takifugu obscurus)响应盐度变化的渗透压调节机制,本研究将淡水养殖的幼鱼转移至盐度34的海水中进行盐度适应,检测了盐度适应不同时间(1、2、3、5、7、10和14 d)后,Na^(+)和Cl^(-)转运蛋白(NHE1)、Na^(+)和葡萄糖转运蛋白(SLC5A1、SLC5A2)、Mg^(2+)转运蛋白(SLC41A1、TRPM6、TRPM7)、Ca^(2+)转运蛋白(Atp2b2)、SO_(4)^(2–)转运蛋白(SLC13A1、SLC26A1)以及水通道蛋白(AQP1、AQP10)这11个渗透压调节相关基因在肾脏和肠道中的表达变化情况。研究结果显示,暗纹东方鲀适应盐度变化的14 d中,NHE1、SLC5A2、SLC41A1、SLC26A1、AQP15个基因的表达变化极其显著,在肾脏中的表达显著升高,最高表达水平可以达到淡水组的30~100倍;但在肠道中,除SLC26A1的表达仍呈上升趋势外,其他4个基因的表达均出现显著的下降,最低下降了40倍。SLC5A1和Atp2b2的表达在肾脏和肠道中均呈现一定程度的上升,但上升倍数不显著。TRPM7的表达在肾脏中上升,肠道中下降,但表达倍数变化不显著。肾脏中TRPM6、SLC13A1和AQP10在淡水中的表达显著高于海水(P<0.05),而肠道中3个基因在海水中的表达则显著高于淡水(P<0.05)。以上表明,暗纹东方鲀在适应盐度变化的过程中,NHE1、SLC5A2、SLC41A1、SLC26A1、AQP15个基因分别作为重要的Na^(+)和Cl^(-)转运蛋白、Na^(+)和葡萄糖转运蛋白、Mg^(2+)转运蛋白、SO_(4)^(2–)转运蛋白和水通道蛋白发挥离子转运功能,且肾脏和肠道中的离子转运模式存在差异。本研究结果从分子水平解析暗纹东方鲀响应盐度变化的渗透压调节机制,也提供了广盐性鱼类生理调控的基础数据。Numerous studies have shown that water salinity impacts fish development and growth.Takifugu obscurus,an anadromous fish of the Yangtze River,regulates osmotic pressure well and is an excellent model organism for studying euryhaline teleosts.The osmotic pressure-regulating organs and tissues of T.obscurus produce a series of physiological responses in reaction to the changes in water salinity during its migratory process.In China,T.obscurus is distributed in the East China Sea,Yellow Sea,Bohai Sea,and inland rivers.Every spring,it spawns along the Yangtze River,and the newly hatched juveniles are fattened in fresh water before returning to the sea at the end of the year or the next spring.In recent years,due to environmental pollution and overfishing,the wild population of T.obscurus has become endangered.The success of artificial propagation has given a significant impetus to the revival of the T.obscurus population.However,there is no systematic research on basic biological issues such as the mechanism of osmotic pressure regulation during its migration.A series of glass tanks with water at different salinities were used to study the changes in osmotic pressure genes in T.obscurus after salinity changes.Feeding was stopped one day before the start of this experiment,and the fish were transferred to a glass tank containing fresh water for 24 h.They were then transferred to 15 salinity artificial seawater and 24 h later they were transferred into water at 34 salinity.Samples were taken from the control group kept in freshwaterand and the 7 experimental groups after 1,2,3,5,7,10,and 14 days of salinity stress respectively.Three fish from each group were randomly selected,and kidney and intestinal tissue samples were taken.The osmolality-related gene expression changes in experimental samples were detected using fluorescence quantitative PCR.H+and Cl^(-)transport protein(NHE1),Na^(+)and glucose transport protein(SLC5A1,SLC5A2),Mg^(2+)transport protein(SLC41A1,TRPM6,TRPM7),Ca^(2+)transporter protein(Atp2b2),SO_(4)^(

关 键 词：暗纹东方鲀 盐度响应 渗透压调节基因 

分 类 号：S917[农业科学—水产科学]