瓦氏雅罗鱼slc26基因家族的鉴定、表达与进化分析  

Identification,expression,and evolution of slc26 gene family in Leuciscus waleckii

作　　者：黄晶 王双毅 李雯 梁利群[2] 张立民[2] 董志国 常玉梅[2] HUANG Jing;WANG Shuangyi;LI Wen;LIANG Liqun;ZHANG Limin;DONG Zhiguo;CHANG Yumei(Jiangsu Key Laboratory of Marine Biological Resources and Environment,Jiangsu Ocean University,Lianyungang 222005,China;National and Local Joint Engineering Laboratory for Freshwater Fish Breeding/Key Laboratory of Freshwater Aquatic Biotechnology and Genetic Breeding,Ministry of Agriculture and Rural Affairs/Heilongjiang Province’s Key Laboratory of Fish Stress Resistance Breeding and Germplasm Characteristics on Special Habitat/Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;BGI Genomics,BGI-Shenzhen,Shenzhen 518083,China;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏连云港222005 [2]中国水产科学研究院黑龙江水产研究所,淡水鱼类育种国家地方联合工程实验室,农业农村部淡水水产生物技术与遗传育种重点实验室,黑龙江省特殊生境鱼类种质特性与抗逆育种重点实验室,黑龙江哈尔滨150070 [3]深圳华大基因股份有限公司,广东深圳518083 [4]上海海洋大学水产与生命学院,上海201306

出　　处：《中国水产科学》2023年第3期309-323,共15页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(32273120);国家重点研发计划项目(2020YFD0900402).

摘　　要：为探究瓦氏雅罗鱼(Leuciscus waleckii)slc26基因家族在Cl^(-)、HCO_(3)^(-)等阴离子转运过程中的作用机制,基于瓦氏雅罗鱼基因组及转录组数据,结合生物信息学方法对slc26家族进行了基因鉴定、表达及进化分析。利用同源比对的方法,从瓦氏雅罗鱼基因组中共鉴定到10个slc26基因家族成员,分布在瓦氏雅罗鱼的6条染色体上,分别命名为slc26a1、slc26a2、slc26a3.1、slc26a3.2、slc26a4、slc26a5、slc26a6、slc26a6l、slc26a10和slc26a611。系统发育和模体分析显示,slc26a3.1、slc26a3.2和slc26a4同源性较高,slc26a5、slc26a6和slc26a6l同源性较高,slc26a1、slc26a2和slc26a10同源性较高,而slc26a11与其他9个基因的同源性相对较低,单独聚类;RNA-seq和实时荧光定量分析显示,50 mmol/L NaHCO3碱度胁迫下瓦氏雅罗鱼slc26家族不同成员呈现出一定的组织表达特异性,即slc26a3.1、slc26a3.2和slc26a4在肠组织高表达,slc26a5、slc26a6和slc26a6l在鳃和脑组织高表达,slc26a1、slc26a10和slc26a11在肾组织高表达,slc26a2在肝脏高表达,亲缘关系与表达结果基本一致。基于基因组重测序数据,统计了slc26基因在瓦氏雅罗鱼碱水种群(23尾)和淡水种群(15尾)的SNPs位点,以Fst top 10%为基准,筛选出12个共享的潜在候选SNPs,通过进一步基因型性状关联分析鉴定出5个SNPs位点在碱水种群和淡水种群存在显著的遗传分化(P<0.05)。本研究结果将为瓦氏雅罗鱼酸碱调控机制的深入解析提供理论依据。The high bicarbonate(HCO_(3)^(-)and CO32–)and pH in alkali-saline water have a significant impact on fish growth,reproduction and survival.Amur ide(Leuciscus waleckii)can survive in Lake Dali,Inner Mongolia,with an alkalinity of more than 54 mmol/L and a pH of 9.6 and become a dominant population.The slc26 gene family has been extensively studied in humans,mice,livestock,and fish and codes for important proteins which regulate the transmembrane transport of Cl^(-)and HCO_(3)^(-).The slc26 gene family in Amur ide was identified to investigate the mechanism of HCO_(3)^(-)transport,and its expression and evolution were studied using genome and transcriptome data from various tissues and populations.As a result,a total of 10 members of the slc26 gene family were identified in Amur ide,which were distributed on 6 chromosomes and named slc26a1,slc26a2,slc26a3.1,slc26a3.2,slc26a4,slc26a5,slc26a6,slc26a6l,slc26a10,and slc26a611.Phylogenetic and motif analysis showed that the clusters of slc26a3.1,slc26a3.2,and slc26a4,had higher homology than those of slc26a5,slc26a6,and slc26a6l,and of slc26a1,slc26a2,and slc26a10,while slc26a11 had lower homology with other nine genes and clustered singly;RNA-seq combined with real-time fluorescence quantitative analysis revealed that with 50 mmol/L bicarbonate stress,different members of the slc26 family in Amur ide had tissue expression specificity.Among them,slc26a3.1,slc26a3.2,and slc26a4 were highly expressed in the intestine;slc26a5,slc26a6,and slc26a6l were highly expressed in the gills and brain;slc26a1,slc26a10,and slc26a11 were highly expressed in the kidney;and slc26a2 was highly expressed in the liver only.The genetic relationships between the slc26 genes were basically consistent with their expression patterns.Based on the genome resequencing data,the SNP loci of slc26 genes of Amur ide in the alkali-adapted population(23 individuals)and freshwater population(15 individuals)were calculated,and 12 potential shared candidate SNPs were identified based on the threshold of t

关 键 词：瓦氏雅罗鱼 盐碱水 slc26基因家族 HCO_(3)^(-)转运 适应性进化 

分 类 号：S917[农业科学—水产科学]