慢性盐度胁迫下金钱鱼卵巢转录组分析  

Ovarian transcriptome analysis in Scatophagus argus under chronic salinity stress

作　　者：潘巧怡 翟毅 蔡博生 江东能 石红娟 黄洋[1,2] 李广丽 邓思平[1,2] PAN Qiaoyi;ZHAI Yi;CAI Bosheng;JIANG Dongneng;SHI Hongjuan;HUANG Yang;LI Guangli;DENG Siping(Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]广东省名特优鱼类生殖调控与繁育工程技术研究中心,广东湛江524088

出　　处：《中国水产科学》2023年第3期324-333,共10页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(31972775).

摘　　要：为了阐明盐度胁迫对金钱鱼(Scatophagus argus)代谢和生殖的影响,采用RNA-seq技术对低盐组(5)、对照组(25)和高盐组(35)处理40 d后的2龄性成熟金钱鱼卵巢进行转录组分析。结果发现,金钱鱼卵巢转录组测序获得raw reads共398681318,clean reads共396910398。从低盐胁迫相对对照组(5 vs 25)和高盐胁迫相对对照组(35 vs 25)中分别筛选到373个和874个差异表达基因(DEGs)。与对照组相比,低盐胁迫后氨基酸代谢相关基因(sds、bhmt)和脂肪酸代谢相关基因(pnpla2)显著下调;高盐胁迫后pgr、cyp17a1和ers1等生殖相关基因显著下调。KEGG通路富集分析发现,低盐胁迫组相对对照组显著富集与代谢相关的通路为半胱氨酸和甲硫氨酸代谢,缬氨酸、亮氨酸和异亮氨酸生物合成代谢,脂肪酸生物合成,脂肪酸代谢,皮质醇的合成和分泌,醛固酮的合成和分泌,脂肪细胞脂解的调节等;高盐胁迫组相对对照组中显著富集的与生殖相关的通路为雌激素信号传导通路。这些结果表明,氨基酸和脂肪酸的代谢调控可能在金钱鱼卵巢的低渗透压调节中起重要作用,高盐胁迫可能抑制了金钱鱼卵巢中性类固醇激素的合成和延迟卵巢成熟发育时间。Salinity is an important environmental factor that can affect the reproduction,growth,development,and metabolism of fish.To clarify the effects of salinity stress on reproduction and metabolism,we studied a two-year-old sexually mature spotted scat(Scatophagus argus).The ovarian transcriptome was analyzed using the RNA-sequence technique after the fish were stressed with low salinity(5),control(25),and high salinity(35)for 40 days.We obtained 398681318 raw reads and 396910398 clean reads from the ovarian transcriptome.A total of 373 and 874 differentially expressed genes(DEGs)were obtained from the low salinity group vs control(5 vs 25)and the high salinity group vs control(35 vs 25),respectively.Compared with the control,the genes related to amino acid metabolism(sds,bhmt)and fatty acid metabolism(pnpla2)were down-regulated with low salt stress,and the reproductive related genes such as pgr,cyp17a1,and ers1 were down-regulated with high salt stress.KEGG pathway enrichment revealed that DEGs were significantly enriched in pathways related to cysteine and methionine metabolism;valine,leucine,and isoleucine biosynthesis;fatty acid biosynthesis;fatty acid metabolism;cortisol synthesis and secretion;aldosterone synthesis and secretion;and regulation of lipolysis in adipocytes with low salinity stress when compared to the control.Compared with the control group,KEGG analysis showed that estrogen signaling pathways were significantly enriched under high salt stress.These results suggest that amino acids and fatty acids are important in metabolic regulation during hypoosmotic pressure adaptation in the ovary.High salt stress may inhibit the synthesis of steroid sex hormones and delay the maturation and development of the ovary in the spotted scat.

关 键 词：金钱鱼 慢性盐度胁迫 转录组测序 差异表达基因 

分 类 号：S917[农业科学—水产科学]