机构地区:[1]College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,People’s Republic of China [2]Jiangsu Co‑Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University,Yangzhou 225009,People’s Republic of China [3]State Key Laboratory of Agrobiotechnology,College of Biological Sciences,China Agricultural University,100193 Beijing,People’s Republic of China [4]Institute of Reproduction and Metabolism,Yangzhou University,225009 Jiangsu,People’s Republic of China
出 处:《Journal of Animal Science and Biotechnology》2023年第3期1040-1053,共14页畜牧与生物技术杂志(英文版)
基 金:supported by the Natural Science Foundation of China (32130098);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)。
摘 要:Background Taurine performs multiple physiological functions,and the maintenance of taurine level for most mammals relies on active uptake from diet and endogenous taurine synthesis through its synthesis enzymes,including cysteine dioxygenase(CDO).In addition,uterus tissue and uterus fluid are rich in to urine,and to urine synthesis is regulated by estrogen(E2)and progesterone(P_(4)),the key hormones priming embryo-uterine crosstalk during embryo implantation,but the functional interactions and mechanisms among which are largely unknown.The present study was thus proposed to identify the effects of CDO and taurine on embryo implantation and related mechanisms by using Cdo knockout(KO)and ovariectomy(OVX)mouse models.Results The uterine CDO expression was assayed from the first day of plugging(d 1)to d 8 and the results showed that CDO expression level increased from d 1 to d 4,followed by a significant decline on d 5 and persisted to d 8,which was highly correlated with serum and uterine taurine levels,and serum P_(4) concentration.Next,Cdo KO mouse was established by CRISPER/Cas9.It was showed that Cdo deletion sharply decreased the taurine levels both in serum and uterus tissue,causing implantation defects and severe subfertility.However,the implantation defects in Cdo KO mice were partly rescued by the taurine supplementation.In addition,Cdo deletion led to a sharp decrease in the expressions of P_(4)receptor(PR)and its responsive genes Ihh,Hoxa10 and Hand2.Although the expression of uterine estrogen receptor(ERa)had no significant change,the levels of ERa induced genes(Muc1,Ltf)during the implantation window were upregulated after Cdo deletion.These accompanied by the suppression of stroma cell proliferation.Meanwhile,E2inhibited CDO expression through ERa and P_(4)upregulated CDO expression through PR.Conclusion The present study firstly demonstrates that taurine and CDO play prominent roles in uterine receptivity and embryo implantation by involving in E2-ERa and P_(4)-PR signaling.These are crucial for our
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