Pterostilbene attenuates intrauterine growth retardation-induced colon inflamm tion in piglets by modulating endoplasmic reticulum stress and autophagy  

作　　者：Yanan Chen Hao Zhang Yue Li Shuli Ji Peilu Jia Tian Wang 

机构地区：[1]College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China [2]Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China.

出　　处：《Journal of Animal Science and Biotechnology》2023年第3期1118-1133,共16页畜牧与生物技术杂志（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China (Nos.31902197 and 31802094);the Natural Science Foundation of Jiangsu Province (No.BK20180531)。

摘　　要：Background:Endoplasmic reticulum(ER)stress and autophagy are implicated in the pathophysiology of intestinal inflammation;however,their roles in intrauterine growth retardation(IUGR)-induced colon inflammation are unclear.This study explored the protective effects of natural stilbene pterostilbene on colon inflammation using the IUGR piglets and the tumor necrosis factor alpha(TNF-α)-treated human colonic epithelial cells(Caco-2)by targeting ER stress and autophagy.Results:Both the IUGR colon and the TNF-α-treated Caco-2 cells exhibited inflammatory responses,ER stress,and impaired autophagic flux(P<0.05).The ER stress inducer tunicamycin and the autophagy inhibitor 3-methyladenine further augmented inflammatory responses and apoptosis in the TNF-α-treated Caco-2 cells(P<0.05).Conversely,pterostilbene inhibited ER stress and restored autophagic flux in the IUGR colon and the TNF-α-treated cells(P<0.05).Pterostilbene also prevented the release of inflammatory cytokines and nuclear translocation of nuclear factor kappa B p65,reduced intestinal permeability and cell apoptosis,and facilitated the expression of intestinal tight junction proteins in the IUGR colon and the TNF-α-treated cells(P<0.05).Importantly,treatment with tunicamycin or autophagosome-lysosome binding inhibitor chloroquine blocked the positive effects of pterostilbene on inflammatory response,cell apoptosis,and intestinal barrier function in the TNF-α-exposed Caco-2 cells(P<0.05).Conclusion:Pterostilbene mitigates ER stress and promotes autophagic flux,thereby improving colon inflammation and barrier dysfunction in the IUGR piglets and the TNF-α-treated Caco-2 cells.

关 键 词：Autophagic flux Colon inflammation Endoplasmic reticulum stress Intrauterine growth retardation PIGLETS 

分 类 号：S858.28[农业科学—临床兽医学]