Effects of arginine replacement with L-citrulline on the arginine/nitric oxide metabolism in chickens:An animal model without urea cycle  

作　　者：Victoria Anthony Uyanga Lijing Sun Yu Liu Meiming Zhang Jingpeng Zhao Xiaojuan Wang Hongchao Jiao Okanlawon M.Onagbesan Hai Lin 

机构地区：[1]Department of Animal Science,College of Animal Science and Veterinary Medicine,Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control,Shandong Agricultural University,No.61 Daizong Street,Tai’an City,Shandong Province 271018,China [2]Department of Animal Physiology,Federal University of Agriculture,Ogun State,Abeokuta P.M.B 2240,Nigeria

出　　处：《Journal of Animal Science and Biotechnology》2023年第3期1182-1200,共19页畜牧与生物技术杂志（英文版）

基　　金：funded by the Key Technologies Research and Development Program of China (2021YFD1300405);Key Technology Research and Development Program of Shandong province (2019JZZY020602);the Earmarked Fund for China Agriculture Research System (CARS-40-K09);National Natural Science Foundation of China (31772619)。

摘　　要：Background This study examined the efficacy of L-citrulline supplementation on the arginine/nitric oxide metabolism,and intestinal functions of broilers during arginine deficiency.A total of 288 day-old Arbor Acre broilers were randomly assigned to either an arginine deficient basal diet(NC diet),NC diet+0.50%L-arginine(PC diet),or NC diet+0.50%L-citrulline(NCL diet).Production performance was recorded,and at 21 days old,chickens were euthanized for tissue collection.Results The dietary treatments did not affect the growth performance of broilers(P>0.05),although NC diet increased the plasma alanine aminotransferase,urate,and several amino acids,except arginine(P<0.05).In contrast,NCL diet elevated the arginine and ornithine concentration higher than NC diet,and it increased the plasma citrulline greater than the PC diet(P<0.05).The nitric oxide concentration in the kidney and liver tissues,along with the plasma and liver e NOS activities were promoted by NCL diet higher than PC diet(P<0.05).In the liver,the activities of arginase 1,ASS,and ASL,as well as,the gene expression of i NOS and OTC were induced by PC diet greater than NC diet(P<0.05).In the kidney,the arginase 1,ASS and ASL enzymes were also increased by PC diet significantly higher than the NC and NCL diets.Comparatively,the kidney had higher abundance of n NOS,ASS,ARG2,and OTC genes than the liver tissue(P<0.05).In addition,NCL diet upregulated(P<0.05)the m RNA expression of intestinal nutrient transporters(EAAT3 and PEPT1),tight junction proteins(Claudin 1 and Occludin),and intestinal mucosal defense(MUC2 and p Ig R).The intestinal morphology revealed that both PC and NCL diets improved(P<0.05)the ileal VH/CD ratio and the jejunal VH and VH/CD ratio compared to the NC fed broilers.Conclusion This study revealed that NCL diet supported arginine metabolism,nitric oxide synthesis,and promoted the intestinal function of broilers.Thus,L-citrulline may serve as a partial arginine replacement in broiler’s diet without detrimental impacts on the performanc

关 键 词：Amino acids ARGININE Broiler chicken CITRULLINE Intestinal health Nutrient transporters Tight junctions 

分 类 号：S831.5[农业科学—畜牧学]