Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem  

作　　者：Congli Hou Linyue Tian Guoli Lian Li-Hai Fan Zheng-Jun Li 

机构地区：[1]College of Chemical Engineering,Fujian Engineering Research Center of Advanced Manufacturing Technology for Fine Chemicals,Fuzhou University,Fuzhou,350108,People’s Republic of China [2]College of Life Science and Technology,Beijing University of Chemical Technology,Beijing,100029,People’s Republic of China [3]Qingyuan Innovation Laboratory,Quanzhou,362801,People’s Republic of China

出　　处：《Synthetic and Systems Biotechnology》2023年第2期235-241,共7页合成和系统生物技术（英文）

基　　金：supported by grants from the National Key R&D Program of China(Nos.2021YFC2103100,2018YFA0900200);Quanzhou City Science&Technology Program of China(No.2021CT006).

摘　　要：Fumarate is a value-added chemical that is widely used in food,medicine,material,and agriculture industries.With the rising attention to the demand for fumarate and sustainable development,many novel alternative ways that can replace the traditional petrochemical routes emerged.The in vitro cell-free multi-enzyme catalysis is an effective method to produce high value chemicals.In this study,a multi-enzyme catalytic pathway comprising three enzymes for fumarate production from low-cost substrates acetate and glyoxylate was designed.The acetyl-CoA synthase,malate synthase,and fumarase from Escherichia coli were selected and the coenzyme A achieved recyclable.The enzymatic properties and optimization of reaction system were investigated,reaching a fumarate yield of 0.34 mM with a conversion rate of 34%after 20 h of reaction.We proposed and realized the conversion of acetate and glyoxylate to fumarate in vitro using a cell-free multi-enzyme catalytic system,thus providing an alternative approach for the production of fumarate.

关 键 词：FUMARATE CELL-FREE Multi-enzyme catalysis ACETATE GLYOXYLATE 

分 类 号：O64[理学—物理化学]