Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin  

作　　者：Peng Zhang Jin-Song Gong Zhi-Hao Xie Chang Su Xiao-Mei Zhang Zhi-Ming Rao Zheng-Hong Xu Jin-Song Shi 

机构地区：[1]Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Life Sciences and Health Engineering,Jiangnan University,Wuxi,214122,PR China [2]National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,School of Biotechnology,Jiangnan University,Wuxi,214122,PR China [3]Jiangsu Provincial Engineering Research Center for Bioactive Product Processing,Jiangnan University,Wuxi,214122,PR China [4]Yixing Institute of Food and Biotechnology Co.,Ltd,Yixing,214200,PR China [5]School of Chemical and Material Engineering,Jiangnan University,Wuxi,214122,PR China

出　　处：《Synthetic and Systems Biotechnology》2023年第2期273-280,共8页合成和系统生物技术（英文）

基　　金：supported by the National Key Research and Development Program of China(No.2021YFC2100900);the National Natural Science Foundation of China(No.32171261);the Natural Science Foundation of Jiangsu Province(No.BK20221082);the Fundamental Research Funds for the Central Universities(No.JUSRP21940).

摘　　要：Phospholipase D(PLD)is an essential biocatalyst for the biological production of phosphatidylserine and phospholipid modification.However,the efficient heterologous expression of PLD is limited by its cell toxicity.In this study,a PLD was secretory expressed efficiently in Bacillus subtilis with an activity around 100 U/mL.A secretory expression system containing the signal peptide SPEstA and the dual-promoter PHpaII-SrfA was estab-lished,and the extracellular PLD activity further reached 119.22 U/mL through scale-up fermentation,191.30-fold higher than that of the control.Under optimum reaction conditions,a 61.61%conversion ratio and 21.07 g/L of phosphatidylserine production were achieved.Finally,the synthesis system of PL derivates was established,which could efficiently synthesis novel PL derivates.The results highlight that the secretory expression system constructed in this study provides a promising PLD producing strain in industrial application,and laid the foundation for the biosynthesis of phosphatidylserine and other PL derivates.As far as we know,this work re-ports the highest level of extracellular PLD expression to date and the enzymatic production of several PL der-ivates for the first time.

关 键 词：Phospholipase D Secretion expression Bacillus subtilis BIOSYNTHESIS Enzymatic PLs modification 

分 类 号：S565.1[农业科学—作物学]