Microbial synthesis of bacitracin:Recent progress,challenges,and prospects  

作　　者：Jiang Zhu Shiyi Wang Cheng Wang Zhi Wang Gan Luo Junhui Li Yangyang Zhan Dongbo Cai Shouwen Chen 

机构地区：[1]State Key Laboratory of Biocatalysis and Enzyme Engineering,Environmental Microbial Technology Center of Hubei Province,College of Life Sciences,Hubei University,Wuhan,430062,PR China [2]Hubei Provincial Key Laboratory of Industrial Microbiology,Key Laboratory of Fermentation Engineering(Ministry of Education),School of Food and Biological Engineering,Hubei University of Technology,Wuhan,430068,Hubei,PR China [3]Lifecome Biochemistry Co.Ltd,Nanping,353400,PR China

出　　处：《Synthetic and Systems Biotechnology》2023年第2期314-322,共9页合成和系统生物技术（英文）

基　　金：supported by National Key Research and Development Program of China(2022YFA0911800);Knowledge Innovation Program of Wuhan-Shuguang Project(2022020801020334);Science and Technology Project of Hubei Tobacco Company(027Y2021-023,027Y2020-013).

摘　　要：Microorganisms are important sources of various natural products that have been commercialized for human medicine and animal healthcare.Bacitracin is an important antibacterial natural product predominantly pro-duced by Bacillus licheniformis and Bacillus subtilis,and it is characterized by a broad antimicrobial spectrum,strong activity and low resistance,thus bacitracin is extensively applied in animal feed and veterinary medicine industries.In recent years,various strategies have been proposed to improve bacitracin production.Herein,we systematically describe the regulation of bacitracin biosynthesis in genus Bacillus and its associated mechanism,to provide a theoretical basis for bacitracin overproduction.The metabolic engineering strategies applied for bacitracin production are explored,including improving substrate utilization,using an enlarged precursor amino acid pool,increasing ATP supply and NADPH generation,and engineering transcription regulators.We also present several approaches of fermentation process optimization to facilitate the industrial large-scale production of bacitracin.Finally,the challenges and prospects associated with microbial bacitracin synthesis are discussed to facilitate the establishment of high-yield and low-cost biological factories.

关 键 词：BACILLUS BACITRACIN Metabolic regulation Metabolic engineering Fermentation process 

分 类 号：O62[理学—有机化学]