家蚕小分子热激蛋白BmHsp19.1基因克隆及在蚕卵中的表达特征  

Cloning and Expression Analysis of BmHsp19.1 Gene in Silkworm Eggs

作　　者：龚竞[1,2] 张伟 唐苗[1] 程悦静 庞家欣 GONG Jing;ZHANG Wei;TANG Miao;CHENG Yuejing;PANG Jiaxin(College of Sericulture,Textile and Biomass Sciences,Southwest University,Chongqing 400715,China;State Key Laboratory of Resource Insects,Chongqing 400715,China)

机构地区：[1]西南大学蚕桑纺织与生物质科学学院,重庆400715 [2]资源昆虫高效养殖与利用全国重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2023年第6期109-115,共7页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(31872429,31802139);重庆市自然科学基金项目(cstc2021jcyj-msxmX1166).

摘　　要：小分子热激蛋白(small heat shock proteins,sHsps)是细胞抵御压力首先反应的蛋白,目前家蚕卵滞育相关的sHsps研究较少.为了更好地了解sHsps在滞育过程中的作用,以转录组筛选出的差异表达基因BmHsp19.1为研究对象,通过生物信息学分析、基因克隆、PCR等方法对其进行克隆鉴定及在滞育卵、非滞育卵等蚕卵中的表达特征进行分析.结果发现:BmHsp19.1基因ORF框全长507 bp,编码168个氨基酸,理论分子量为19.06 kDa,有典型的Hsp20结构域,属于sHsps家族;qRT-PCR结果显示该基因在滞育卵3~7 d表达量上调,而在非滞育卵中仅在第7 d上调表达,且滞育卵中表达量显著高于非滞育卵,暗示BmHsp19.1基因在蚕卵进入滞育时可能发挥作用,起到了保护机体免受损伤的抗逆作用.结果为解析BmHsp19.1基因功能提供了有用的信息,也为其他昆虫的sHsps研究提供了一些有价值的参考.Small heat shock proteins(sHsps)are the first line of cell defense.However,to date,the sHsps of Bombyx mori(BmsHsps),which are related to diapause of silkworm eggs,were not well studied.In order to better understand the role of BmsHsps in the process of diapause,the BmHsp19.1 gene,which is differentially expressed between diapause eggs and nondiapause eggs in the transcriptome,was studied by gene cloning,bioinformatics methods and PCR analysis.The results showed that the ORF of the BmHsp19.1 gene was 507 bp in length,encoding a total 168 amino acids,and the theoretical molecular weight of the protein was 19.06 kDa,which had a typical Hsp20 domain and belonged to the sHsps family.The BmHsp19.1 was up-regulated at day 3 to 7 after oviposition in diapause eggs,while it was up-regulated only at day 7 after oviposition in nondiapause eggs.Moreover,the expression level of BmHsp19.1 in diapause eggs was significantly higher than that in nondiapause eggs.It is speculated that BmHsp19.1 gene is involved in silkworm enter diapause,and plays an anti-stress role in protecting the body from injury.The results increased our understanding in the biological functions of BmHsp19.1,and may facilitate the sHsps study of other insects.

关 键 词：家蚕 BmHsp19.1基因 滞育 基因克隆 

分 类 号：S882[农业科学—特种经济动物饲养]