3D-printed fish gelatin scaffolds for cartilage tissue engineering  

作　　者：Abudureheman Maihemuti Han Zhang Xiang Lin Yangyufan Wang Zhihong Xu Dagan Zhang Qing Jiang 

机构地区：[1]State Key Laboratory of Pharmaceutical Biotechnology,Division of Sports Medicine and Adult Reconstructive Surgery,Department of Orthopedic Surgery,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,321 Zhongshan Road,Nanjing,210008,Jiangsu,PR China [2]Branch of National Clinical Research Center for Orthopedics,Sports Medicine and Rehabilitation,PR China [3]Co-innovation Center of Neuroregeneration,Nantong University,PR China [4]Department of Rheumatology and Immunology,Nanjing Drum Tower Hospital,School of Biological Science and Medical Engineering,Southeast University,Nanjing,210096,PR China [5]Department of Rheumatology and Immunology,Institute of Translational Medicine,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing,210002,PR China

出　　处：《Bioactive Materials》2023年第8期77-87,共11页生物活性材料（英文）

基　　金：supported by the Key Program of NSFC(81730067);Major Project of NSFC(81991514);Jiangsu Provincial Key Medical Center Foundation,Jiangsu Provincial Medical Outstanding Talent Foundation,Jiangsu Provincial Medical Youth Talent Foundation,and Jiangsu Provincial Key Medical Talent Foundation.The Fundamental Research Funds for the Central Universities(14380493,14380494);the National Natural Science Foundation of China(82102511);the Natural Science Foundation of Jiangsu(BK20210021);Research Project of Jiangsu Province Health Committee(M2021031).

摘　　要：Knee osteoarthritis is a chronic disease caused by the deterioration of the knee joint due to various factors such as aging,trauma,and obesity,and the nonrenewable nature of the injured cartilage makes the treatment of osteoarthritis challenging.Here,we present a three-dimensional(3D)printed porous multilayer scaffold based on cold-water fish skin gelatin for osteoarticular cartilage regeneration.To make the scaffold,cold-water fish skin gelatin was combined with sodium alginate to increase viscosity,printability,and mechanical strength,and the hybrid hydrogel was printed according to a pre-designed specific structure using 3D printing technology.Then,the printed scaffolds underwent a double-crosslinking process to enhance their mechanical strength even further.These scaffolds mimic the structure of the original cartilage network in a way that allows chondrocytes to adhere,proliferate,and communicate with each other,transport nutrients,and prevent further damage to the joint.More importantly,we found that cold-water fish gelatin scaffolds were nonimmunogenic,nontoxic,and biodegradable.We also implanted the scaffold into defective rat cartilage for 12 weeks and achieved satisfactory repair results in this animal model.Thus,cold-water fish skin gelatin scaffolds may have broad application potential in regenerative medicine.

关 键 词：3D printing Fish skin gelatin Sodium alginate Cartilage defect repair Tissue engineering 

分 类 号：R318.08[医药卫生—生物医学工程]