Dual stimulus responsive borosilicate glass(BSG)scaffolds promote diabetic alveolar bone defectsrepair by modulating macrophage phenotype  

作　　者：Pengfei Tian Limin Zhao Jua Kim Xian Li Chunyu Liu Xu Cui Tao Liang Yunbo Du Xiehui Chen Haobo Pan 

机构地区：[1]Center for Human Tissues and Organs Degeneration,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,PR China [2]Shenzhen Longhua District Central Hospital,Shenzhen,518000,PR China [3]Shenzhen Healthemes Biotechnology Co.Ltd,Shenzhen,518102,PR China

出　　处：《Bioactive Materials》2023年第8期231-248,共18页生物活性材料（英文）

基　　金：supported by the National Key R&D Program of China(2021YFC2400500);National Natural Science Foundation of China(Nos.U2001221 and 32161160327);Shenzhen Science and Technology Program(JCYJ20200109114620793 and JSGGKQTD20210831174330015);Guangdong Basic and Applied Basic Research Foundation(2022B 1515120048 and 2023A1515011727).

摘　　要：The regeneration of alveolar bone is still clinical challenge,particularly accompanied with diabetes,causing metabolic disorder with a protracted low-grade inflammatory phenotype.As a result,the anticipated loading of biomaterials is highly suspicious in spontaneous modulation of cells function,which is mostly disturbed by constant inflammation.In this study,we developed glucose and hydrogen peroxide dual-responsive borosilicate glass(BSG)scaffolds loaded with epigallocatechin gallate(EGCG)to synergistically modulate the abnormal inflammation of diabetic alveolar bone defects.It was found that the release of EGCG by BSG could directly regulate the shift of macrophages from M1 to the M2 phenotype by promoting autophagy and lessening the inhibition of autophagic flux.Moreover,EGCG can also indirectly regulate the polarization phenotype of macrophages by reducing the activation of NF-κb in stem cells and restoring its immunoregulatory capacity.Therefore,the addition of EGCG to BSG scaffold in diabetes allows for a more striking modulation of the macrophage phenotype in a timely manner.The altered macrophage phenotype reduces local inflammation and thus increases the ability to repair diabetic alveolar bone,showing promise for the treatment of alveolar defect in diabetic patients.

关 键 词：BOROSILICATE Epigallocatechin gallate(EGCG) Alveolar bone Autophagy Mitochondria Macrophage immunomodulatory Diabetes 

分 类 号：R587.1[医药卫生—内分泌] R318.08[医药卫生—内科学]