Single-cell protein-DNA interactomics and multiomics tools for deciphering genome regulation  

作　　者：Haiqing Xiong Runyu Wang Aibin He 

机构地区：[1]State Key Laboratory of Experimental Hematology,National Clinical Research Center for Blood Diseases,Haihe Laboratory of Cell Ecosystem,Institute of Hematology&Blood Diseases Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300020,China [2]Peking University-Tsinghua University-National Institute of Biological Science Joint Graduate Program,Academy for Advanced Interdisciplinary Studies,Peking University,Beijing 100871,China [3]Institute of Molecular Medicine,College of Future Technology,Peking-Tsinghua Center for Life Sciences,Peking University,Beijing 100871,China

出　　处：《National Science Open》2023年第3期64-82,共19页国家科学进展（英文）

基　　金：supported by the National Key Research and Development Program of China(2021YFA1100101 and 2017YFA0103402);the National Natural Science Foundation of China(32025015 and 31771607);the Peking-Tsinghua Center for Life Sciences.

摘　　要：The emergence of single-cell genomic and transcriptomic sequencing accelerates the development of single-cell epigenomic technologies,providing an unprecedented opportunity for decoding cell fate decisions largely encoded in the epigenome.Recent advances in single-cell multimodality epigenomic technologies facilitate directly interrogating the reg-ulatory relationship between multi-layer molecular information in the same cell.In this review,we discuss recent progress in development of single-cell multimodality epigenomic technologies and applications in elucidating cellular diversifications in development and diseases,with a focus on protein-DNA interactomics and regulatory links between epigenome and tran-scriptome.Further,we provide perspective on the future direction of single-cell multiomics tool development as well as challenges facing ahead.

关 键 词：single-cell protein-DNA interaction single-cell multiomics single-cell epigenomics epigenome genome regulation 

分 类 号：Q75[生物学—分子生物学]