反相HPLC快速测定全反式维生素A含量的方法探究  

Rapid Determination of All Trans Vitamin A by Reversed Phase HPLC

作　　者：温恺嘉 王小妹 林元亨[1,2,3] 许文东 韩亚明 袁诚[1,2,3] 梁北梅 黄晓玲[1,2,3] 武林贺 卢嘉颉[1,2,3] WEN Kaijia;WANG Xiaomei;LIN Yuanheng;XU Wendong;HAN Yaming;YUAN Cheng;LIANG Beimei;HUANG Xiaoling;WU Linhe;LU Jiajie(Guangzhou Hanfang Pharmaceutical Co.,Ltd.,Guangzhou 510240,China;National Engineering Research Center of Pharmaceutical Processing Technology of Traditional Chinese Medicine and Drug Innovation,Guangzhou 510240,China;Guangdong Provincial Key Laboratory of Medicinal Lipid,Guangzhou 510240,China)

机构地区：[1]广州白云山汉方现代药业有限公司,广东广州510240 [2]中药制药过程技术与新药创制国家工程研究中心,广东广州510240 [3]广东省药用脂质重点实验室,广东广州510240

出　　处：《现代食品》2023年第7期157-162,共6页Modern Food

基　　金：广东省科技计划项目“广东省药用脂质重点实验室”(2020B1212070024);2020年重大科技创新产业项目“基于临床营养的脂质原辅料及特医食品研发及产业化”。

摘　　要：目的:建立了反相高效液相色谱法(High Performance Liquid Chromatography,HPLC)测定全反式维生素A含量,采用碘试液反应生成的全反式维生素A棕榈酸酯及其异构体在本方法条件下分离度为1.62,检测方法专属性好。方法:本方法前处理简单,利用涡旋振荡器提取目标成分,再利用反相HPLC进行反式维生素A的定量测定。结果:适用于全反式维生素A及其异构体的快速分离检测。结论:对主要含维生素A棕榈酸酯的食品原料及其制剂中该成分的含量检测具有指导性作用。Objective:A reversed phase high performance liquid chromatography method was established to determine the content of all trans vitamin A.Under the conditions of this method,the resolution of all trans vitamin A palmitate and its isomers produced by the reaction of iodine test solution was 1.62,and the specificity of the method was good.Method:The method was simple in pretreatment.The target components were extracted by vortex oscillator,and then the quantitative determination of trans vitamin A was carried out by reversed-phase HPLC.Result:It is suitable for the rapid separation and detection of all trans vitamin A and its isomers.Conclusion:It is instructive for the content determination of vitamin A palmitate in food raw materials and preparations.

关 键 词：反式维生素A 维生素A棕榈酸酯 反相液相色谱 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]