禁食和复投喂对大口黑鲈胆囊收缩素及其受体基因表达的影响  

FASTING AND REFEEDING ON THE EXPRESSION OF CHOLECYSTOKININ AND ITS RECEPTOR GENE IN LARGEMOUTH BASS

作　　者：邵嘉棋 李胜杰[1] 杜金星 董传举 雷彩霞 宋红梅[1] 姜鹏[1] SHAO Jia-Qi;LI Sheng-Jie;DU Jin-Xing;DONG Chuan-Ju;LEI Cai-Xia;SONG Hong-Meiand JIANG Peng(Key Laboratory of Aquatic Genomics,Ministry of Agriculture,Key Laboratory of Tropical&Subtropical Fishery Resource Application&Cultivation of Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences,Guangzhou 510380,China;College of Fisheries,Henan Normal University,Xinxiang 453007,China)

机构地区：[1]中国水产科学研究院珠江水产研究所,农业农村部热带亚热带水产资源利用与养殖重点实验室,广州510380 [2]河南师范大学水产学院,新乡453007

出　　处：《水生生物学报》2023年第8期1220-1227,共8页Acta Hydrobiologica Sinica

基　　金：中国水产科学研究院基本科研业务费(2022CG02)资助。

摘　　要：为研究大口黑鲈(Micropterus salmoides)胆囊收缩素(Cholecystokinin,CCK)和其受体(Cholecystokinin receptor,CCKR)基因在摄食活动中的功能,研究通过克隆得到CCK1、CCK2、CCK1R和CCK2R基因的编码区序列,其长度分别为414、387、1368和1359bp,分别编码137、128、455和452个氨基酸。荧光定量结果表明CCK1和CCK2基因均在脑组织中高表达,其次为肠道组织,而CCK1R和CCK2R基因分别在胆囊和脑组织中高表达。在摄食后24h内,CCK1、CCK2、CCK1R和CCK2R基因的相对表达量呈先升高后下降趋势,其中CCK1、CCK1R和CCK2R基因在摄食后3h相对表达量达到最高值,而CCK2基因在摄食后12h相对表达量达到最高值(P<0.05)。禁食过程中CCK1、CCK1R和CCK2R基因相对表达量在禁食14d时显著升高(P<0.05)。复投喂后CCK1、CCK1R和CCK2R基因的相对表达趋势与餐后表达趋势相似,呈先升高后降低趋势。但在禁食和复投喂过程中CCK2基因相对表达量并无显著变化。综上所述,研究结果推测CCK1基因可能与CCK1R、CCK2R基因结合,作为饱腹信号因子,通过抑制食欲调控大口黑鲈摄食、消化等生理过程;而CCK2基因可能作为短期食欲因子调节摄食活动。研究结果可为大口黑鲈摄食活动调节提供理论依据。CCK as brain-gut peptide,binds to its receptor(CCKR)and participates in physiological processes such as feeding and digestion in animals.In order to study the function of CCKs and CCKRs in largemouth bass(Micropterus salmoides)in feeding activities,the coding sequences of CCK1,CCK2,CCK1R and CCK2R were cloned in this study.Their lengths were 414,387,1368 and 1359 bp,and their encodes were 137,128,455 and 452 amino acids,respectively.Fluorescence quantitative results showed that both CCK1 and CCK2 were highly expressed in brain,followed by intestinal,while CCK1R and CCK2R were highly expressed in gallbladder and brain,respectively.Within 24h after ingestion,the relative expressions of CCK1,CCK2,CCK1R and CCK2R increased first and then decreased.The relative expression of CCK1,CCK1R and CCK2R reached the highest value at 3h after ingestion,while the relative expression of CCK2 was at 12h after ingestion.The relative expression reached the highest value(P<0.05).During fasting,the relative expression of CCK1,CCK1R and CCK2R was significantly increased on the 14th day of fasting(P<0.05).The relative expression trends of CCK1,CCK1R and CCK2R after refeeding were similar to those after meals,showing a trend of first increasing and then decreasing.However,there was no significant change in the relative expression of CCK2 during fasting and refeeding.In conclusion,the results of this study indicated that CCK1 can be combined with CCK1R and CCK2R to act as a satiety signal factor to regulate physiological processes such as feeding and digestion of largemouth bass by suppressing appetite;CCK2 may act as a short-term appetite factor to regulate feeding.Activity.The results of this study can provide a theoretical basis for the regulation of feeding activities of largemouth bass.

关 键 词：胆囊收缩素 胆囊收缩素受体 摄食 禁食复投喂 饱腹信号因子 食欲调控 大口黑鲈 

分 类 号：S965.1[农业科学—水产养殖]