光唇鱼仔稚幼鱼肠道菌群与养殖水体细菌群落的相关性  

CORRELATION BETWEEN THE INTESTINAL MICROBIOTA OF LARVAL AND JUVENILE ACROSSOCHEILUS FASCIATUS AND BACTERIAL COMMUNITY OF CULTURE WATER

作　　者：李珊珊 张伟佳 高阳 储张杰 李海东 LI Shan-Shan;ZHANG Wei-Jia;GAO Yang;CHU Zhang-Jie;LI Hai-Dong(School of Fishery,Zhejiang Ocean University,Zhoushan 316022,China;Key Laboratory of Marine Fishery Equipment and Technology of Zhejiang,Zhoushan 316022,China)

机构地区：[1]浙江海洋大学水产学院,舟山316022 [2]浙江省海洋渔业装备技术研究重点实验室,舟山316022

出　　处：《水生生物学报》2023年第8期1313-1322,共10页Acta Hydrobiologica Sinica

基　　金：浙江省教育厅一般项目(Y202147681);浙江省海水增养殖重点实验室开放课题(2021KF002)资助。

摘　　要：采用16S rRNA高通量测序,系统研究了光唇鱼(Acrossocheilus fasciatus)仔鱼、稚鱼和幼鱼肠道菌群组成及与同时期养殖水体细菌群落的相关性。研究结果表明,光唇鱼仔鱼、稚鱼和幼鱼的肠道菌群中Chao1指数和Shannon指数均没有显著变化(P>0.05);而随着光唇鱼幼体的发育,养殖水体中Chao1指数和Shannon指数呈现显著下降趋势(P<0.05)。光唇鱼仔鱼和稚鱼肠道菌群中的优势菌门由变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)组成,而同时期养殖水体中优势菌门为变形菌门;光唇鱼幼鱼肠道菌群中的优势菌门为梭杆菌门(Fusobacteria)和变形菌门,同时期养殖水体中优势菌门由变形菌门、拟杆菌门和梭杆菌门组成。线性回归分析结果显示,随光唇鱼幼体发育,在光唇鱼肠道菌群中变形菌门、厚壁菌门和梭杆菌门相对丰度的时序变化趋势与其在养殖水体中相同。在属水平上,光唇鱼仔鱼、稚鱼肠道菌群中优势菌属均为醋酸杆菌属(Acetobacter),而幼鱼肠道菌群中优势菌属为鲸杆菌属(Cetobacterium)。稀有菌群和条件稀有菌群是光唇鱼肠道菌群和养殖水体细菌群落中的主要细菌分类类群。相关性分析表明,光唇鱼肠道菌群与养殖水体细菌群落组成相关性不显著(P>0.05)。研究揭示了光唇鱼仔鱼、稚鱼、幼鱼肠道菌群及养殖水体中细菌群落的组成、结构及演替变化,为深入研究光唇鱼仔稚幼鱼肠道菌群及健康养殖提供了参考依据。Acrossocheilus fasciatus has been main economical fish in the mountain areas of Zhejiang Province because of its high economic value and increasing demand.Based on 16S rRNA high-throughput sequencing,we systematically studied the correlation between intestinal microbiota of larval and juvenile Acrossocheilus fasciatus and bacterial community of culture water within the same period.The results showed that Chao1 and Shannon indices had no significant changes in intestinal microbiota of larval and juvenile A.fasciatus(P>0.05);however,the Chao1 and Shannon indices of culture water showed a significant downward trend with the development of A.fasciatus(P<0.05).ANOSIM analysis revealed that the compositions of intestinal microbiota in the larval and juvenile A.fasciatus were significantly different from the bacterial community of culture water(P<0.05).The dominant phyla were Proteobacteria,Bacteroidetes and Firmicutes in the intestinal microbiota of larval A.fasciatus,while Proteobacteria was the dominant phylum in culture water within the same period.Fusobacteria and Proteobacteria were the dominant phyla in the intestinal microbiota of juvenile A.fasciatus,while the dominant phylum in culture water within the same period consisted of Proteobacteria,Bacteroidetes and Fusobacteria.Linear regression analysis revealed that the changes of relative abundance of Proteobacteria,Firmicutes and Fusobacteria were consistent in the A.fasciatus and culture water.At the genus level,Acetobacter was the dominant genus in the intestinal microbiota of larval A.fasciatus,while Cetobacterium was the dominant genus in juvenile A.fasciatus.Rare taxa and conditional rare taxa were the main taxa in the intestinal microbiota of A.fasciatus and bacterial community of culture water.Correlation analysis showed that no significant correlation was observed between intestinal microbiota of A.fasciatus and bacterial community of culture water(P>0.05).SourceTracker analysis verified that the proportional contributions of culture water to shaping the

关 键 词：肠道菌群 养殖水体 细菌群落 光唇鱼 仔稚幼鱼 

分 类 号：Q178.1[生物学—水生生物学]