健脾化湿通络方对佐剂型关节炎滑膜成纤维细胞凋亡和炎症影响的研究  

Effect of Jianpi Huashi Tongluo Formula on Apoptosis and Inflammation of Synovial Fibroblasts in Adjuvant Arthritis

作　　者：刘剑桥 张明媚 刘晓闯[1] 霍星星[1] 姜娟娟 李传润 LIU Jianqiao;ZHANG Mingmei;LIU Xiaochuang;HUO Xingxing;JIANG Juanjuan;LI Chuanrun(Grade Three-level Laboratory of TCM Preparation,State Administration of Traditional Chinese Medicine,the First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,China;School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230013,China)

机构地区：[1]安徽中医药大学第一附属医院国家中医药管理局中药制剂三级实验室,安徽合肥230031 [2]安徽中医药大学药学院,安徽合肥230013

出　　处：《现代中药研究与实践》2023年第2期47-51,共5页Research and Practice on Chinese Medicines

基　　金：国家自然科学基金(81804043);安徽省教育厅科研基金(KJ2020A0399)。

摘　　要：目的探讨健脾化湿通络方含药血清对佐剂型关节炎大鼠滑膜成纤维细胞(FLSs)凋亡和炎症影响。方法大鼠足趾皮下注射弗氏完全佐剂复制佐剂型关节炎大鼠模型,分离培养原代FLSs;按10倍临床等效剂量(3.24 mg/g),每日2次,连续灌胃给药3 d制备含药血清。CCK-8评价健脾化湿通络方对FLSs增殖能力影响;流式细胞术检测健脾化湿通络方对FLSs凋亡影响;ELISA检测IL-1β、IL-4、IL-10炎症因子水平。结果模型组FLSs凋亡细胞数减少,增殖率上升,IL-1β、IL-4水平上升,IL-10水平下降;健脾化湿通络方含药血清干预后增加FLSs凋亡细胞数,抑制FLSs增殖率,降低IL-1β、IL-4表达水平,提升IL-10表达水平。结论健脾化湿通络方含药血清在体外能有效抑制佐剂型关节炎大鼠FLSs增殖同时促进凋亡,增强抑炎因子表达,降低炎症因子水平。Objective To investigate the effect of Jianpi Huashi Tongluo Formula-containing serum on apoptosis and inflammation of synovial fibroblasts(FLSs)in rats with adjuvant arthritis.Methods The primary FLSs was isolated and cultured in the rat model of adjuvant arthritis by subcutaneous injection of Fredrich’s complete adjuvant.10 times the clinical equivalent dose(3.24 mg/g)was given twice a day for 3 d by gavage to prepare the drug-containing serum.Cell CCK-8 was used to evaluate the effect of Jianpi Huashi Tongluo Formula on the proliferation of FLSs.Flow cytometry was used to detect the effect of Jianpi Huashi Tongluo Formula on the apoptosis of FLSs.ELISA was used to detect the levels of IL-1β,IL-4 and IL-10 inflammatory factors.Results The number of apoptotic cells in FLSs decreased.However,the proliferation rate of FLSs increased.The levels of IL-1βand IL-4 increased and the level of IL-10 decreased significantly.The serum containing Jianpi Huashi Tongluo Formula significantly increased the number of FLSs apoptotic cells,inhibited the proliferation rate of FLSs cells,decreased the expression levels of IL-1βand IL-4,and increased the expression level of IL-10.Conclusion Jianpi Huashi Tongluo Formula can effectively inhibit the proliferation of FLSs in adjuvant arthritis rats in vitro,promote apoptosis and enhance the expression of anti-inflammatory factors and reduce the level of inflammatory factors.

关 键 词：健脾化湿通络方 佐剂型关节炎 滑膜成纤维细胞 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]