检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:王丽晖 黄旭东 汪晶华 张超 史永红 杨新军 WANG Lihui;HUANG Xudong;WANG Jinghua;ZHANG Chao;SHI Yonghong;YANG Xinjun(Department of Nephrology,the 980th Hospital of PLA Joint Logistics Support Forces Bethune International Peace Hospital,Shijiazhuang 050082,China)
机构地区:[1]中国人民解放军联勤保障部队第九八〇医院(白求恩国际和平医院)肾脏病科,石家庄050082
出 处:《临床误诊误治》2023年第5期32-37,共6页Clinical Misdiagnosis & Mistherapy
基 金:河北省医学科学研究课题计划(20211725);2022年度联勤保障部队第九八〇医院科技孵育计划立项课题(FMJMHS⁃07)。
摘 要:目的探讨达格列净对高糖培养肾小球系膜细胞丝裂原活化蛋白激酶磷酸酶⁃1(MKP⁃1)表达的影响及其与结缔组织生长因子(CTGF)表达和细胞外基质蛋白分泌的关系。方法体外培养大鼠肾小球系膜HBZY⁃1细胞,分为达格列净干预组(30 mmol/L葡萄糖+10μmol/L达格列净培养)、高糖组(30 mmol/L葡萄糖培养)、渗透压对照组(5.5 mmol/L葡萄糖+24.5 mmol/L甘露醇培养)、正常对照组(5.5 mmol/L葡萄糖培养)。各组细胞培养48 h后,采用Western blotting检测MKP⁃1、p38丝裂原活化蛋白激酶(MAPK)及p⁃p38 MAPK蛋白的表达,RT⁃PCR检测CTGF和纤维连接蛋白(FN)mRNA的表达。ELISA法测定细胞上清液CTGF和FN含量,放射免疫法测定细胞上清液Ⅳ型胶原的含量。结果与正常对照组比较,高糖组MKP⁃1蛋白表达降低,CTGF和p⁃p38 MAPK蛋白表达升高,CTGF和FN mRNA表达升高,细胞上清液CTGF、FN和Ⅳ型胶原含量增加(P<0.05,P<0.01)。与高糖组比较,达格列净干预组MKP⁃1表达升高,CTGF、p⁃p38 MAPK蛋白表达降低,CTGF和FN mRNA表达降低,细胞上清液CTGF、FN和Ⅳ型胶原含量降低(P<0.05)。结论达格列净可以有效抑制高糖培养肾小球系膜细胞CTGF表达和细胞外基质蛋白分泌,可能与激活MKP⁃1后,增强p38 MAPK去磷酸化有关。Objective To investigate the effect of Dagliazine on the expression of mitogen⁃activated protein kinase phosphatase⁃1(MKP⁃1)in mesangial cells cultured with high glucose and its relationship with the expression of connective tis⁃sue growth factor(CTGF)and secretion of extracellular matrix protein.Methods Rat mesangial cells HBZY⁃1 cultured in vitro were divided into three groups:Dagliazine intervention group(30 mmol/L glucose+10μmol/L Dagliazine culture),high glucose group(30 mmol/L glucose culture),osmotic pressure control group(5.5 mmol/L glucose+24.5 mmol/L man⁃nitol culture)and normal control group(5.5 mmol/L glucose culture).At 48 h after cell culture,the expressions of MKP⁃1,p38 mitogen activated protein kinase(MAPK)and p⁃p38MAPK proteins were detected by Western blotting,and the mRNA expressions of CTGF and fibronectin(FN)were detected by RT⁃PCR.The contents of CTGF and FN in cell supernatant were determined by ELISA,and the contents of typeⅣcollagen in cell supernatant were determined by radioimmunoassay.Results Compared with the normal control group,the protein expression of MKP⁃1 was decreased,while protein expression of CTGF and p⁃p38 MAPK,and mRNA expression of CTGF and FN in the high glucose group,as well as the contents of CTGF,FN and typeⅣcollagen in the supernatant of cells were increased(P<0.05,P<0.01).Compared with high glucose group,MKP⁃1 expression was increased,while CTGF and p⁃p38 MAPK protein expression,CTGF and FN mRNA expression,as well as CTGF,FN and typeⅣcollagen contents in supernatants of cells were decreased in Dagliazine intervention group(P<0.05).Conclusion Dagliazine can effectively inhibit CTGF expression and extracellular matrix protein secretion in mesangial cells cultured with high glucose,which may be related to enhanced dephosphorylation of p38 MAPK after activation of MKP⁃1.
关 键 词:肾小球系膜细胞 糖尿病肾病 达格列净 丝裂原活化蛋白激酶磷酸酶⁃1 结缔组织生长因子 丝裂原活化蛋白激酶 纤维连接蛋白 Ⅳ型胶原
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.56